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Gene Expression Data
Assay Details
Assay
Reference: J:192970 Lee MB, et al., Betaine homocysteine methyltransferase is active in the mouse blastocyst and promotes inner cell mass development. J Biol Chem. 2012 Sep 21;287(39):33094-103
Assay type: RT-PCR
MGI Accession ID: MGI:6385063
Gene symbol: Bhmt
Gene name: betaine-homocysteine methyltransferase
Probe: Bhmt-pA, Bhmt-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Validated by confirming expected expression pattern of the previously established control gene Ppia.
Results
Image: 2B Bhmt ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 218 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
gO P9 TS28: oocyte Ambiguous Not Specified CF-1 Female growing oocyte.
GV P w 4-7 TS28: primary oocyte Ambiguous Not Specified CF-1 Female germinal vesicle stage.
MII P w 4-7 TS28: meiotic germ cell of ovary Ambiguous Not Specified CF-1 Female mature egg.
1c E0.5 (a) TS1: 1-cell stage conceptus Absent Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified one-cell stage.
2c E1.0 (a) TS2: 2-cell stage conceptus Trace Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified two-cell stage.
4c E2.0 (a) TS3: 4-cell stage conceptus Present Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified four-cell stage.
8c E2.5 (a) TS3: 8-cell stage conceptus Present Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified eight-cell stage.
M E3.0 (a) TS4: morula-stage conceptus Strong Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified morula stage.
B E3.5 (a) TS5: blastocyst-stage conceptus Present Not Specified either: (involves: 129S2/SvPasCrl * CF-1) or (involves: C57BL/6 * CF-1 * DBA/2) Not Specified blastocyst stage.
W Control
L P adult TS28: liver Present Not Specified Not Specified Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory