Probe:	T beta 4 probe1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: oral epithelium	Strong	Regionally restricted	1B	Strong expression was seen in the oral mucosal epithelial layer at the site where the tooth germ would form.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Strong	Regionally restricted	1D	Strong expression was seen in the oral mucosal epithelial layer at the site where the tooth germ would form.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar epithelium	Present		1F
TS22: oral epithelium	Present	Regionally restricted	1F	Expression was detected in the mucosal epithelial layer.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar epithelium	Present		1H
TS22: oral epithelium	Present	Regionally restricted	1H	Expression was detected in the mucosal epithelial layer.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar enamel organ	Strong	Regionally restricted	1J	Strong expression was detected in the outer enamel epithelium. Weak expression signal was detected in the inner enamel epithelium.
TS22: dental lamina	Strong		1J
TS22: oral epithelium	Strong		1J
TS22: enamel knot	Present		1J	Expression was detected in the primary enamel knot.

Structure	Level	Pattern	Image	Note
TS23: lower jaw molar enamel organ	Strong	Regionally restricted	1L	Strong expression was detected in the outer enamel epithelium.
TS23: enamel knot	Absent		1L

Structure	Level	Pattern	Image	Note
TS24: lower jaw molar enamel organ	Strong	Non-Uniform	1N	Strong signal was partly expressed in the outer enamel epithelium, and a weak expression was found in the inner enamel epithelial cells.
TS24: dental lamina	Strong		1N
TS24: dental papilla	Absent		1N	No expression was detected in dental papilla, except for the blood vessels.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Strong	Non-Uniform	1P	Strong signal was partly expressed in the outer enamel epithelium, and a weak expression was found in the inner enamel epithelial cells.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Strong	Regionally restricted	2B	Strong expression was detected throughout the inner enamel epithelial layer. Weak expression was seen in the outer enamel epithelium.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Strong	Regionally restricted	2D	Strong expression was detected in the inner enamel epithelial layer.
TS27: late bell stage tooth	Strong	Regionally restricted	2H , 2I , 2J , 2D	Expression was reduced in the ameloblasts in the lingual cusp with matrix formation. Strong expression was detected in the preameloblasts in the central groove region and the buccal cusp site. No in situ signals were found in preodontoblasts or odontoblasts on P1.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Present	Regionally restricted	2O	Expression was located in the preameloblasts at the lateral side of the tooth germ. Weak expression was detected in the preameloblasts and ameloblasts in the inner enamel epithelium.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Present	Regionally restricted	2Q	Expression was located in the preameloblasts at the lateral edges of the inner enamel epithelium. No signal was detected in the preodontoblasts or odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar crown	Trace		3C	There was almost no expression in the crown at this stage.

Structure	Level	Pattern	Image	Note
TS28: molar crown	Trace		3F	There was almost no expression in the crown at this stage.

Structure	Level	Pattern	Image	Note
TS28: molar crown	Absent		3I
TS28: molar root	Absent		3I