Probe:	T beta 10 probe1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	1A	Diffuse expression was detected in the subepithelial mesenchymal cells. Strong expression was observed in mesenchymal cells that aggregated beneath the mucosal epithelium.
TS17: oral epithelium	Weak	Regionally restricted	1A	Weak expression was detected in the oral mucosal epithelial cells.

Structure	Level	Pattern	Image	Note
TS20: mandibular process mesenchyme	Strong	Regionally restricted	1C	Diffuse expression was detected in the subepithelial mesenchymal cells. Strong expression was observed in mesenchymal cells that aggregated beneath the mucosal epithelium.
TS20: oral epithelium	Weak	Regionally restricted	1C	Weak expression was detected in the oral mucosal epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar enamel organ	Weak	Regionally restricted	1E	Weak expression was detected in the epithelial cells in the central area of the tooth bud.
TS22: lower jaw molar mesenchyme	Strong	Regionally restricted	1E	Strong expression was observed in the mesenchymal cells that surrounded the invaginated epithelial tooth bud.
TS22: lower jaw mesenchyme	Weak	Diffuse	1E

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar mesenchyme	Strong	Regionally restricted	1G	Strong expression was detected in the mesenchymal cells surrounding the epithelial tooth bud.
TS22: lower jaw molar epithelium	Weak	Regionally restricted	1G	Weak expression was detected in the epithelial cells in the central area of the tooth bud.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar dental papilla	Strong		1I
TS22: lower jaw molar enamel organ	Strong	Regionally restricted	1I	Strong expression was localized to the outer enamel epithelial cells and inner cells of the enamel organ.
TS22: dental sac	Weak		1I	Weak signal in the mesenchymal cells of the dental sac.
TS22: enamel knot	Absent		1I	No signal was detected in the primary enamel knot.

Structure	Level	Pattern	Image	Note
TS23: lower jaw molar dental papilla	Strong		1K
TS23: lower jaw molar enamel organ	Strong	Regionally restricted	1K	Strong expression was localized to the outer enamel epithelial cells and inner cells of the enamel organ.
TS23: dental sac	Weak		1K	Weak signal in the mesenchymal cells of the dental sac.
TS23: enamel knot	Absent		1K	No signal was detected in the primary enamel knot.

Structure	Level	Pattern	Image	Note
TS24: lower jaw molar dental papilla	Strong	Regionally restricted	1M	Strong expression was detected in the dental papilla at the presumptive cusps.
TS24: lower jaw molar enamel organ	Weak	Regionally restricted	1M	Weak expression was detected in the inner enamel epithelium. No expression was seen in the outer enamel epithelium.
TS24: dental sac	Weak		1M
TS24: oral epithelium	Absent		1M

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar dental papilla	Strong	Regionally restricted	1O	Strong expression was detected in the dental papilla at the presumptive cusps.
TS26: lower jaw molar enamel organ	Weak	Regionally restricted	1O	Weak expression was detected in the inner enamel epithelium.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Present	Regionally restricted	2A	Expression was detected in the inner and outer enamel epithelial cells.
TS27: dental pulp	Present	Regionally restricted	2A	Expression was detected in preodontoblasts in the lingual cusp site of the dental pulp.

Structure	Level	Pattern	Image	Note
TS27: late bell stage tooth	Strong	Regionally restricted	2F , 2C , 2G , 2E	Strong expression was detected in the preodontoblasts and preameloblasts in the buccal cusp site and in the preodontoblasts and preameloblasts in the central groove region. Expression was reduced in the odontoblasts and ameloblasts in the lingual cusp.

Structure	Level	Pattern	Image	Note
TS27: late bell stage tooth	Strong	Regionally restricted	2N	Strong expression was detected in preodontoblasts and preameloblasts at the lateral side of the tooth germ. Weak expression was found in the preodontoblasts located at the presumptive cusps and the occlusal groove sites.
TS27: dental pulp	Strong	Regionally restricted	2N	Strong expression was detected in the lateral sides of the dental pulp.
TS27: lower jaw molar enamel organ	Strong	Regionally restricted	2N	Strong expression was detected in the inner and outer enamel epithelia at the lateral sides of the enamel organ.

Structure	Level	Pattern	Image	Note
TS27: late bell stage tooth	Strong	Regionally restricted	2P	Strong expression was detected in preodontoblasts and preameloblasts at the lateral side of the tooth germ.

Structure	Level	Pattern	Image	Note
TS28: dental pulp	Strong	Regionally restricted	3B	Strong expression was detected in preodontoblasts in a limited area of the lateral side of the tooth germ.
TS28: lower jaw molar enamel organ	Strong	Regionally restricted	3B	Strong expression was detected in the Hertwigs epithelial root sheath.

Structure	Level	Pattern	Image	Note
TS28: dental pulp	Strong	Regionally restricted	3E	Strong expression was detected in preodontoblasts in a limited area of the lateral side of the tooth germ.
TS28: lower jaw molar enamel organ	Strong	Regionally restricted	3E	Strong expression was detected in the Hertwigs epithelial root sheath.

Structure	Level	Pattern	Image	Note
TS28: dental pulp	Present	Regionally restricted	3H	Expression was detected in dental pulp cells facing the Hertwigs epithelial root sheath.
TS28: lower jaw molar enamel organ	Present	Regionally restricted	3H	Expression was detected in the Hertwigs epithelial root sheath.
TS28: periodontal ligament	Present		3H