Antibody:	Anti-Mef2a
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5_left	Staining was nuclear.
TS28: cerebellum internal granule cell layer	Present		5_left	Staining was nuclear.
TS28: cerebellum external granule cell layer	Weak	Diffuse	5_left

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		6_Adult
TS28: cerebellum internal granule cell layer	Present		6_Adult
TS28: cerebellum molecular layer	Present		6_Adult

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		6_P7	Staining was nuclear.
TS28: cerebellum internal granule cell layer	Present		6_P7	Staining was nuclear.
TS28: cerebellum external granule cell layer	Weak	Diffuse	6_P7

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present		8_Thalamus

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Strong		8_Hippocampus
TS28: CA2 pyramidal cell layer	Strong		8_Hippocampus
TS28: CA3 pyramidal cell layer	Strong		8_Hippocampus
TS28: dentate gyrus granule cell layer	Strong		8_Hippocampus
TS28: hippocampus CA4	Strong		8_Hippocampus

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong		8_Olfactory Bulb
TS28: main olfactory bulb mitral cell layer	Strong		8_Olfactory Bulb

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		8_Cortex	Expression was detected in all layers of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: olfactory tubercle	Strong
TS28: main olfactory bulb granule cell layer	Strong			High expression in both the external and internal granule cell layer.
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb glomerular layer	Absent
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: dentate gyrus	Moderate
TS28: entorhinal cortex	Moderate
TS28: subiculum	Moderate
TS28: CA1 pyramidal cell layer	Strong
TS28: CA2 pyramidal cell layer	Strong
TS28: CA3 pyramidal cell layer	Strong
TS28: hippocampus CA4	Strong
TS28: cerebral cortex	Strong
TS28: cerebellum Purkinje cell layer	Very strong
TS28: cerebellum granule cell layer	Strong
TS28: cerebellum molecular layer	Moderate
TS28: deep cerebellar nucleus	Moderate
TS28: brainstem	Trace
TS28: interpeduncular nucleus	Moderate
TS28: red nucleus	Moderate
TS28: facial nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Moderate
TS28: inferior colliculus	Strong
TS28: lateral mammillary nucleus	Weak
TS28: hypothalamic nucleus	Weak	Regionally restricted		Low expression in the lateral nucleus (hypothalamus).
TS28: amygdala	Strong
TS28: telencephalon septum	Strong
TS28: anterior thalamic group	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: thalamic nucleus	Present	Regionally restricted		Medium expression in the habenular nucleus. High expression in the medial nuc. group and lateral nuc. group.
TS28: anterior pretectal nucleus	Trace
TS28: zona incerta	Trace
TS28: caudate nucleus	Moderate
TS28: putamen	Moderate
TS28: substantia nigra	Moderate
TS28: globus pallidus	Weak
TS28: subthalamic nucleus	Weak