Probe:	HMG-17 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Regionally restricted	2A/B	Expression concentrated in differentiating hepatocytes. Hardly any expression in hematopoietic cells.
TS22: intestine epithelium	Present		2A/B
TS22: intestine mesenchyme	Present		2A/B
TS22: pancreas epithelium	Present		2A/B
TS22: pancreas mesenchyme	Present		2A/B

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Uniform	2C/D	Evenly distributed expression.
TS22: bronchiole	Strong	Regionally restricted	2C/D	Abundant expression in branches of bronchioles.
TS22: lung mesenchyme	Strong	Regionally restricted	2C/D	Abundant expression in lung mesenchymal cells and branches of bronchioles. No expression in mesenchymal cells surrounding central branch of bronchiole.

Structure	Level	Pattern	Image	Note
TS25: nephrogenic zone	Strong	Regionally restricted	3A/B	Abundant expression in cortical collecting ducts and nephrogenic region, which contains undifferentiated mesenchyme and differetiating epithelial structures, such as comma- and S-shaped bodies.
TS25: cortical early proximal tubule	Weak		3A/B	Expression dowregulated in more mature structures, such a proximal tubules and glomeruli.
TS25: maturing glomerular tuft	Weak		3A/B	Expression dowregulated in more mature structures, such a proximal tubules and glomeruli.

Structure	Level	Pattern	Image	Note
TS25: atrium cardiac muscle	Present	Uniform	3C/D	Evenly expressed in ventricular and atria myocardium and endocardium.
TS25: atrium endocardial lining	Present	Uniform	3C/D	Evenly expressed in ventricular and atria myocardium and endocardium.
TS25: ventricle cardiac muscle	Present	Uniform	3C/D	Evenly expressed in ventricular and atria myocardium and endocardium.
TS25: ventricle endocardial lining	Present	Uniform	3C/D	Evenly expressed in ventricular and atria myocardium and endocardium.
TS25: atrioventricular valve	Present	Regionally restricted	3C/D	Strong expression in endocardial cells lining atrioventricular valve. Weak expression in myocardium of valve.

Structure	Level	Pattern	Image	Note
TS25: respiratory bronchiole epithelium	Strong		3E/F
TS25: terminal bronchiole epithelium	Strong		3E/F
TS25: alveolar duct	Weak		3E/F	Weak expression in cells of alveolar ducts and alveolar sacs.
TS25: lung alveolus	Weak		3E/F	Weak expression in cells of alveolar ducts and alveolar sacs.
TS25: alveolar septum	Weak	Regionally restricted	3E/F	Weak expression in interalveolar septa, except at periphery of lung, where expression was abundant.

Structure	Level	Pattern	Image	Note
TS25: bronchiole epithelium	Strong		3G	High level of expression in ciliated columnar cells lining bronchioles.
TS25: alveolus epithelium	Weak		3G	Expression declines in region where cuboidal cells of bronchioles abrupty changes into flattened epithelial cells llining alveolar sacs.
TS25: alveolar septum	Weak		3G

Structure	Level	Pattern	Image	Note
TS25: bronchiole epithelium	Strong		3H	High level of expression in ciliated columnar cells lining bronchioles.

Structure	Level	Pattern	Image	Note
TS25: lung alveolus	Weak		3I	Expression declines in region where cuboidal cells of bronchioles abrupty changes into flattened epithelial cells llining alveolar sacs.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present	Regionally restricted	4A/B	Expression in developing crypts between villi.
TS25: intestine mesenchyme	Present		4A/B	Expression in underlying muscular and connective tissue layers.
TS25: intestine muscularis	Present		4A/B	Expression in underlying muscular and connective tissue layers.

Structure	Level	Pattern	Image	Note
TS25: liver	Present	Regionally restricted	4C/D	Expression concentrates in differentiating hepatocytes. Weak expression in cells surrounding hepatic vein. Hardly any expression in hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS25: spleen	Present	Regionally restricted	4E/F	Expression concentrates in lymphatic cells. Hardly any expression in hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS25: tunica albuginea of testis	Present		4G/H
TS25: testis mesenchyme	Present	Regionally restricted	4G/H	Expression in underlying condensing mesenchymal cells.
TS25: interstitium of the testis	Present		4G/H
TS25: seminiferous cord	Present	Regionally restricted	4G/H	Expression in seminipherous tubules.