Probe:	Shh probe158
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		1A	Expression in whisker buds.

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		1B	Expression in whisker buds.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	1C	Expression restricted to rostral whisker buds.
TS22: hair	Present	Punctate	1C	Expression in hair buds of dorsal region.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	1D	Expression restricted to rostral whisker buds.

Structure	Level	Pattern	Image	Note
TS22: hair	Present	Regionally restricted	1F	Expression in hair buds of developing limb.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	2E	Expression in epidermal regions which will become the whisker placode.

Structure	Level	Pattern	Image	Note
TS22: vibrissa placode	Present		2F	Expression continues in the whisker placode.

Structure	Level	Pattern	Image	Note
TS22: vibrissa placode	Present		2G	Expression continues in the whisker placode.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2H	Expression region shifts to basal region in contact with the primordium of the dermal papilla.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2I	Expression region shifts to basal region in contact with the primordium of the dermal papilla.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2J	Expression region shifts to basal region in contact with the primordium of the dermal papilla.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2K	Expression region shifts to basal region in contact with the primordium of the dermal papilla.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2L	Expression region shifts to basal region in contact with the primordium of the dermal papilla.

Structure	Level	Pattern	Image	Note
TS25: vibrissa bulb	Present	Regionally restricted	2N	Expression in whisker bulb surrounding dermal papilla.

Structure	Level	Pattern	Image	Note
TS25: vibrissa bulb	Present	Regionally restricted	2O	Expression in whisker bulb surrounding dermal papilla.

Structure	Level	Pattern	Image	Note
TS21: upper jaw incisor	Present		3A	Expression in incisors of mandible.

Structure	Level	Pattern	Image	Note
TS21: palatal rugae	Present		3B	Expression in prominent rugae of maxillary plate.

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor	Present		3C
TS22: lower jaw molar	Present		3C
TS22: thyroid gland	Present		3C	Expression in thyroid primordium.

Structure	Level	Pattern	Image	Note
TS22: upper jaw incisor	Present		3D
TS22: upper jaw molar	Present		3D
TS22: palatal rugae	Present		3D	Expression in prominent rugae on maxillary plate.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Regionally restricted	3E	In cap stage of first molar, expression localized to cuspal region of enamel organ, the enamel knot.
TS22: enamel knot	Present	Regionally restricted	3E	In cap stage of first molar, expression localized to cuspal region of enamel organ, the enamel knot.

Structure	Level	Pattern	Image	Note
TS25: bell stage tooth	Present	Regionally restricted	3F	In bell stage of first molar, expression region shifted to ameloblasts.

Structure	Level	Pattern	Image	Note
TS22: thyroid gland	Present	Regionally restricted	3G	Expression in epithelial region of thyroid primordium.

Structure	Level	Pattern	Image	Note
TS22: palatal rugae epithelium	Present		3H	Expression in epithelial region of prominent rugae.