Probe:	Reln probe6
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex marginal layer	Present	Regionally restricted	3C	Expression is in a single row of cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex marginal layer	Present	Regionally restricted	3H	Expression is similar to wild-type.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present	Regionally restricted	3M	Expression is in a single row of cells.
TS22: neocortex	Present	Regionally restricted	3M	Expression is in two rows of cells which correspond to migrating interneurons.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present	Regionally restricted	3R	Expressing cells are less densely packed and form small clusters in the dorsomedial cortex in this mutant compared to wild-type.
TS22: neocortex	Present	Regionally restricted	3R	Expression is in rows of cells in the lateral neocortex while more medial regions showed an uneven distribution.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex marginal zone	Strong	Regionally restricted	4A' , 4A	Expression is in Cajal Retzius cells.
TS27: cortical layer IV	Present	Single cells	4A , 4A'
TS27: cortical layer V	Present	Single cells	4A , 4A'

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex marginal zone	Weak	Single cells	4B' , 4B
TS27: cortical layer IV	Weak	Single cells	4B , 4B'	In this mutant there is a more dispersed distribution of expressing cells.
TS27: cortical layer V	Weak	Single cells	4B , 4B'	In this mutant there is a more dispersed distribution of expressing cells.