Antibody:	anti-CPNE7
Detection system:	Secondary antibody coupled to biotin

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present		2A
TS20: tooth bud	Present		2A
TS20: mandibular process mesenchyme	Absent		2A

Structure	Level	Pattern	Image	Note
TS23: cap stage tooth	Present		2B
TS23: lower jaw molar epithelium	Present		2B
TS23: dental sac	Weak		2B
TS23: lower jaw molar mesenchyme	Absent		2B

Structure	Level	Pattern	Image	Note
TS26: bell stage tooth	Present		2C , 2C'
TS26: lower jaw molar enamel organ	Present		2C , 2C'
TS26: lower jaw molar mesenchyme	Present	Single cells	2C' , 2C	Expression is in a few mesenchymal cells neighboring inner enamel epithelium.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	2D' , 2D	Expression is in preameloblasts.
TS26: lower jaw molar dental papilla	Strong	Regionally restricted	2D , 2D'	Expression is in preodontoblasts.
TS26: late bell stage tooth	Strong	Regionally restricted	2D , 2D'

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar enamel organ	Weak	Regionally restricted	3C , 3B , 3A	Expression is in mature ameloblasts.
TS28: molar dental pulp	Present	Regionally restricted	3A , 3B , 3C	Expression is in odontoblasts.
TS28: dentin	Present		3B , 3C , 3A
TS28: predentin	Present		3B , 3C , 3A

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Strong		3D , 3F , 3E
TS28: predentin	Present		3D , 3F , 3E
TS28: molar crown	Present	Regionally restricted	3D , 3E	Expression is in the odontoblast process of crown dentin.
TS28: dentin	Present	Regionally restricted	3D , 3F , 3E	Expression is in the odontoblast process of crown dentin.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Strong		3G , 3H , 3I
TS28: predentin	Present		3H , 3I , 3G
TS28: molar crown	Present	Regionally restricted	3H , 3G	Expression is in the odontoblast process of crown dentin.
TS28: dentin	Present	Regionally restricted	3G , 3I , 3H	Expression is in the odontoblast process of crown dentin.

Structure	Level	Pattern	Image	Note
TS28: dentin	Present	Regionally restricted	5C , 5A , 5B	Expression is in forming root dentin and is weak in crown dentin.
TS28: predentin	Present	Regionally restricted	5C , 5A , 5B	Expression is in forming root dentin and is weak in crown dentin.
TS28: molar dental pulp	Present	Regionally restricted	5C , 5B , 5A	Expression is in differentiating odontoblasts in the root.

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar	Absent		5F , 5E , 5D