Probe:	Isl1 probe31
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present	Regionally restricted		Expression in facial branchiomotor (fbm) neurons. From E10, fbm cell bodies start migrating from rhombomere 4 (r4).

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Present	Regionally restricted	1C	Expression in fbm neurons migrating from rhombomere 4 (r4) through r5 to r6.
TS19: hindbrain floor plate	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	1E	Expression in fbm neurons all along migratory stream. Expression in neurons that have finished migrating and started to form the facial nucleus in rhombomere 6. First fbm neurons reach final destination detected at E12.5.
TS20: hindbrain floor plate	Absent		1E

Structure	Level	Pattern	Image	Note
TS22: facial nucleus	Present	Regionally restricted	1G	Facial motor nucleus migration is completed around E14.5. Fbm neurons start to segregate into motor neuron spools called sub-nuclei.

Structure	Level	Pattern	Image	Note
TS24: facial nucleus	Present	Regionally restricted	1I	Fbm neurons segregate into motor neuron spools called sub-nuclei. Expression in all fbm neurons during sub-nuclei segregation from E14.5 to E18.5.

Structure	Level	Pattern	Image	Note
TS26: facial nucleus	Present	Regionally restricted	1K	Fbm neurons segregate into motor neuron spools called sub-nuclei. Expression in all fbm neurons during sub-nuclei segregation from E14.5 to E18.5.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		3I

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		3J	Authors report no difference in mutant compared to control embryos.