Gene Expression Data
Assay Details
Assay
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Reference:
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J:36716
Tajbakhsh S, et al., Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature. 1996 Nov 21;384(6606):266-70
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6381457
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2a left:
embryonic day 9.75; Myf5tm1Pas/Myf5 +
(more )
Specimen
2a left:
(close )
Genetic Background:
|
Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
|
embryonic day 9.75
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Sex:
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Not Specified
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Type:
|
whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS16: somite |
Present |
Regionally restricted |
2a left
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Positive myotomal cells accumulate under the dermomyotome.
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TS16: myotome |
Present |
|
2a left
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Specimen
2a right:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
|
embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS16: somite |
Present |
Non-Uniform |
2a right
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In rostral somites where the segmental organization of muscle cells was lost, and at thoracic levels where a ventral accumulation was also observed
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Specimen
2b:
embryonic day 9.75; Myf5tm1Pas/Myf5 +
(more )
Note: Resin section
Specimen
2b:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Resin/plastic
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Note:
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Resin section
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Structure |
Level |
Pattern |
Image |
Note |
TS16: somite |
Present |
Regionally restricted |
2b
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Positive myotomal cells accumulate under the dermomyotome.
|
TS16: myotome |
Present |
|
2b
|
|
Specimen
2c:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
|
embryonic day 9.75
|
Sex:
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Not Specified
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Type:
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section
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Embedding:
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Resin/plastic
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Note:
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Resin section
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Structure |
Level |
Pattern |
Image |
Note |
TS16: somite |
Present |
Regionally restricted |
2c
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Like those in the wild-type, positive muscle progenitors delaminated from the dermomyotome to become mesenchymal, but migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.
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Specimen
4a:
embryonic day 10.75; Myf5tm1Pas/Myf5 +
(more )
Specimen
4a:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
|
Myf5tm1Pas/Myf5+
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Age:
|
embryonic day 10.75
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4b:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
|
embryonic day 10.75
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS18: somite |
Present |
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4b
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Most dramatic disorganization of positive progenitors in the rostral somites.
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Specimen
4c:
embryonic day 10.75; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: blue - Myf5; dark grains - Twist2 (assay MGI:6381459).
Specimen
4c:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Myf5; dark grains - Twist2 (assay MGI:6381459).
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Specimen
4d/e:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
|
Double labeled: blue - Myf5; dark grains - Twist2 (assay MGI:6381459).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: somite |
Present |
Regionally restricted |
4d
,
4e
|
Like those in the wild-type, positive muscle progenitors delaminated from the dermomyotome to become mesenchymal, but migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.
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Specimen
5a:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: blue - Myf5; dark grains - Scx (assay MGI:6381460).
Specimen
5a:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Myf5; dark grains - Scx (assay MGI:6381460).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: myotome |
Present |
Regionally restricted |
5a
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Expressed in the intercostal muscles, distinct from the Scx hybridization signal.
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Specimen
5b/c:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Myf5; dark grains - Scx (assay MGI:6381460).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: somite |
Present |
|
5c
,
5b
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Positive cells were dispersed among the condensing cells of the ribs. Many of them expressed Scx.
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Specimen
6a:
embryonic day 16.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
6a:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
6b:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: rib |
Present |
Regionally restricted |
6b
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Expressed in the central part of the rib anlagen.
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TS24: intercostal muscle |
Present |
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6b
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The muscles in this region were perturbed.
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Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: somite |
Present |
Regionally restricted |
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At caudal levels, positive cells migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.
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