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Gene Expression Data
Assay Details
Assay
Reference: J:36716 Tajbakhsh S, et al., Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature. 1996 Nov 21;384(6606):266-70
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6381457
Gene symbol: Myf5
Gene name: myogenic factor 5
Reporter: lacZ
Direct Detection
Results
Specimen 2a left: embryonic day 9.75; Myf5tm1Pas/Myf5+ (more )
Structure Level Pattern Image Note
TS16: somite Present Regionally restricted 2a left Positive myotomal cells accumulate under the dermomyotome.
TS16: myotome Present 2a left

Specimen 2a right: embryonic day 9.75; Myf5tm1Pas/Myf5tm1Pas (more )
Structure Level Pattern Image Note
TS16: somite Present Non-Uniform 2a right In rostral somites where the segmental organization of muscle cells was lost, and at thoracic levels where a ventral accumulation was also observed

Specimen 2b: embryonic day 9.75; Myf5tm1Pas/Myf5+ (more )
Note: Resin section
Structure Level Pattern Image Note
TS16: somite Present Regionally restricted 2b Positive myotomal cells accumulate under the dermomyotome.
TS16: myotome Present 2b

Specimen 2c: embryonic day 9.75; Myf5tm1Pas/Myf5tm1Pas (more )
Note: Resin section
Structure Level Pattern Image Note
TS16: somite Present Regionally restricted 2c Like those in the wild-type, positive muscle progenitors delaminated from the dermomyotome to become mesenchymal, but migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.

Specimen 4a: embryonic day 10.75; Myf5tm1Pas/Myf5+ (more )
Structure Level Pattern Image Note
TS18: myotome Present 4a

Specimen 4b: embryonic day 10.75; Myf5tm1Pas/Myf5tm1Pas (more )
Structure Level Pattern Image Note
TS18: somite Present 4b Most dramatic disorganization of positive progenitors in the rostral somites.

Specimen 4c: embryonic day 10.75; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: blue - Myf5; dark grains - Twist2 (assay MGI:6381459).
Structure Level Pattern Image Note
TS18: myotome Present 4c

Specimen 4d/e: embryonic day 10.75; Myf5tm1Pas/Myf5tm1Pas (more )
Note: Double labeled: blue - Myf5; dark grains - Twist2 (assay MGI:6381459).
Structure Level Pattern Image Note
TS16: somite Present Regionally restricted 4d , 4e Like those in the wild-type, positive muscle progenitors delaminated from the dermomyotome to become mesenchymal, but migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.

Specimen 5a: embryonic day 12.5; Myf5tm1Pas/Myf5+ (more )
Note: Double labeled: blue - Myf5; dark grains - Scx (assay MGI:6381460).
Structure Level Pattern Image Note
TS20: myotome Present Regionally restricted 5a Expressed in the intercostal muscles, distinct from the Scx hybridization signal.

Specimen 5b/c: embryonic day 12.5; Myf5tm1Pas/Myf5tm1Pas (more )
Note: Double labeled: blue - Myf5; dark grains - Scx (assay MGI:6381460).
Structure Level Pattern Image Note
TS20: somite Present 5c , 5b Positive cells were dispersed among the condensing cells of the ribs. Many of them expressed Scx.

Specimen 6a: embryonic day 16.5; Myf5tm1Pas/Myf5+ (more )
Structure Level Pattern Image Note
TS24: rib Absent 6a
TS24: intercostal muscle Present 6a

Specimen 6b: embryonic day 16.5; Myf5tm1Pas/Myf5tm1Pas (more )
Structure Level Pattern Image Note
TS24: rib Present Regionally restricted 6b Expressed in the central part of the rib anlagen.
TS24: intercostal muscle Present 6b The muscles in this region were perturbed.

Specimen not shown: embryonic day 12.5; Myf5tm1Pas/Myf5tm1Pas (more )
Structure Level Pattern Image Note
TS20: somite Present Regionally restricted At caudal levels, positive cells migrated aberrantly. Some ventrally in the sclerotome, whereas other cells became positioned dorsally, adjacent to the ectoderm, in regions that do not normally contain myogenic cells.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory