Gene Expression Data
Assay Details
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Assay
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Reference:
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J:279204
Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6380649
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Gene symbol:
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Usp48
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Gene name:
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ubiquitin specific peptidase 48
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Reporter:
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FLAG
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Antibody:
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Anti-Flag tag antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
5A:
(close  )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine crypt of lieberkuhn |
Present |
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5B
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Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5C:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cecum |
Present |
Regionally restricted |
5C
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Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5D:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5Q:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5R:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: small intestine crypt of lieberkuhn |
Present |
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5R
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Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5S:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cecum |
Present |
Regionally restricted |
5S
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Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5T:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
7A:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Strong |
Ubiquitous |
7A
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Specimen
7B:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: embryo |
Moderate |
Non-Uniform |
7B
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Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.
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Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: embryo |
Present |
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7C
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Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.
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Specimen
9 USP48.3xFLAG:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spleen |
Present |
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9_right upper left
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Analysis Tools
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