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Gene Expression Data
Assay Details
Assay
Reference: J:279204 Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6380512
Gene symbol: Usp48
Gene name: ubiquitin specific peptidase 48
Antibody: USP48 Antibody, A301-190A
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 5E: postnatal week 13; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS28: gallbladder epithelium Present Widespread 5E

Specimen 5F: postnatal week 13; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS28: small intestine crypt of lieberkuhn Present 5F Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Specimen 5G: postnatal week 13; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS28: cecum Present Regionally restricted 5G Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Specimen 5H: postnatal week 13; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5H

Specimen 5M: postnatal week 13 (more )
Structure Level Pattern Image Note
TS28: gallbladder epithelium Present 5M

Specimen 5N: postnatal week 13 (more )
Structure Level Pattern Image Note
TS28: small intestine crypt of lieberkuhn Present 5N Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Specimen 5O: postnatal week 13 (more )
Structure Level Pattern Image Note
TS28: cecum Present Regionally restricted 5O Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.

Specimen 5P: postnatal week 13 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5P

Specimen 7D: embryonic day 12.5; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS20: embryo Strong Ubiquitous 7D

Specimen 7E: embryonic day 15.5; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS23: embryo Moderate Non-Uniform 7E Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.

Specimen 7F: embryonic day 18.5; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS26: embryo Present 7F Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.

Specimen 7J: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: embryo Strong Ubiquitous 7J

Specimen 7K: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: embryo Moderate Non-Uniform 7K Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.

Specimen 7L: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: embryo Present 7L Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.

Specimen not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: epidermis Present
TS26: ganglion Present
TS26: intestine Present
TS26: salivary gland Present
TS26: thymus Present
TS26: brain Present Non-Uniform Heterogeneous expression.
TS26: connective tissue Present Non-Uniform Heterogeneous expression.
TS26: liver Moderate Moderate to weak expression.
TS26: lung Moderate Moderate to weak expression.

Specimen not shown: embryonic day 18.5; Usp48tm1.1Dfre/Usp48tm1.1Dfre (more )
Structure Level Pattern Image Note
TS26: epidermis Present
TS26: ganglion Present
TS26: intestine Present
TS26: salivary gland Present
TS26: thymus Present
TS26: brain Present Non-Uniform Heterogeneous expression.
TS26: connective tissue Present Non-Uniform Heterogeneous expression.
TS26: liver Moderate Moderate to weak expression.
TS26: lung Moderate Moderate to weak expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory