Gene Expression Data
Assay Details
Assay
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Reference:
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J:279204
Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6380512
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Gene symbol:
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Usp48
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Gene name:
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ubiquitin specific peptidase 48
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Antibody:
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USP48 Antibody, A301-190A
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
5E:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5F:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: small intestine crypt of lieberkuhn |
Present |
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5F
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Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5G:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cecum |
Present |
Regionally restricted |
5G
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Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5H:
(close )
Genetic Background:
|
involves: C57BL/6NCrl
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Mutant Allele(s):
|
Usp48tm1.1Dfre/Usp48tm1.1Dfre
|
Age:
|
postnatal week 13
|
Sex:
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Not Specified
|
Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5M:
postnatal week 13
(more )
Specimen
5M:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
5N:
postnatal week 13
(more )
Specimen
5N:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 13
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: small intestine crypt of lieberkuhn |
Present |
|
5N
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Nuclear expression in the cells of the small intestinal crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5O:
postnatal week 13
(more )
Specimen
5O:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cecum |
Present |
Regionally restricted |
5O
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Nuclear expression in the cells of the crypts as well as basolateral enterocytes with decreasing signal intensity as the cells differentiated and migrated up the villi.
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Specimen
5P:
postnatal week 13
(more )
Specimen
5P:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 13
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
7D:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: embryo |
Strong |
Ubiquitous |
7D
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Specimen
7E:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: embryo |
Moderate |
Non-Uniform |
7E
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Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.
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Specimen
7F:
(close )
Genetic Background:
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involves: C57BL/6NCrl
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Mutant Allele(s):
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Usp48tm1.1Dfre/Usp48tm1.1Dfre
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: embryo |
Present |
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7F
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Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.
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Specimen
7J:
embryonic day 12.5
(more )
Specimen
7J:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: embryo |
Strong |
Ubiquitous |
7J
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Specimen
7K:
embryonic day 15.5
(more )
Specimen
7K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: embryo |
Moderate |
Non-Uniform |
7K
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Moderate to weak expression in most tissues, although there was diminished immunoreactivity in the liver, brain and connective tissue.
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Specimen
7L:
embryonic day 18.5
(more )
Specimen
7L:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: embryo |
Present |
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7L
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Expressed in the ganglia, salivary gland, intestine, epidermis and thymus. Also heterogeneous detection in the brain and connective tissues, and moderate to weak expression in the liver and lung.
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Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
|
Embedding:
|
Paraffin
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6NCrl
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Mutant Allele(s):
|
Usp48tm1.1Dfre/Usp48tm1.1Dfre
|
Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Formalin
|
Embedding:
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Paraffin
|
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