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Gene Expression Data
Assay Details
Assay
Reference: J:127540 Margagliotti S, et al., The Onecut transcription factors HNF-6/OC-1 and OC-2 regulate early liver expansion by controlling hepatoblast migration. Dev Biol. 2007 Nov 15;311(2):579-89
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6379992
Gene symbol: Hnf4a
Gene name: hepatic nuclear factor 4, alpha
Antibody: anti-HNF-4alpha
Detection system: Alexa Fluor 594
Results
Specimen 2G: embryonic day 9.0 (more )
Note: 18 somites. Control embryos were either wild-type or Hnf6+/+;Oc2+/lacZ.
Structure Level Pattern Image Note
TS14: hepatic primordium Present 2G
Specimen 2H: embryonic day 9.0; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: 18 somites. Hnf6/Oc2-deficient embryos were either Hnf6-/-;Oc2+/lacZ or Hnf6-/-; Oc2lacZ/lacZ.
Structure Level Pattern Image Note
TS14: hepatic primordium Present 2H Expression not affected compared to controls.
Specimen 2O: embryonic day 9.5 (more )
Note: 25 somites. Control embryos were either wild-type or Hnf6+/+;Oc2+/lacZ. Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS15: hepatic primordium Present 2O
Specimen 2P: embryonic day 9.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: 25 somites. Hnf6/Oc2-deficient embryos were either Hnf6-/-;Oc2+/lacZ or Hnf6-/-; Oc2lacZ/lacZ. Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS15: hepatic primordium Present 2P Expression not affected compared to controls.
Specimen 3D: embryonic day 9.0; Onecut2tm1Flem/Onecut2+ (more )
Note: 20 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS14: hepatic primordium Present 3D
TS14: septum transversum Present 3D
Specimen 3E: embryonic day 9.0; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2+ (more )
Note: 20 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS14: hepatic primordium Present 3E
TS14: septum transversum Present 3E
Specimen 3F: embryonic day 9.0; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: 21 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS15: hepatic primordium Present 3F
TS15: septum transversum Ambiguous 3F
Specimen 3G: embryonic day 9.5; Onecut2tm1Flem/Onecut2+ (more )
Note: 27 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS15: hepatic primordium Present 3G
TS15: septum transversum Present 3G
Specimen 3H: embryonic day 9.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2+ (more )
Note: 26 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS15: hepatic primordium Present 3H
Specimen 3I: embryonic day 9.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: 26 somites. Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS15: septum transversum Ambiguous 3I Authors report a reduction in hepatoblasts invading the septum transversum compared to controls.
Specimen 4G: embryonic day 11.5; Onecut2tm1Flem/Onecut2+ (more )
Structure Level Pattern Image Note
TS19: liver Present 4G
Specimen 4H: embryonic day 11.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2+ (more )
Structure Level Pattern Image Note
TS19: liver Present 4H
TS19: septum transversum Present 4H
Specimen 4K: embryonic day 11.5; Onecut2tm1Flem/Onecut2+ (more )
Note: Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS19: liver Present 4K
Specimen 4L: embryonic day 11.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2+ (more )
Note: Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS19: liver Present Clusters 4L Hepatoblast colonize liver lobes, but form clusters.
Specimen 4M: embryonic day 11.5; Onecut2tm1Flem/Onecut2+ (more )
Note: Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS19: liver Present 4M
Specimen 4N: embryonic day 11.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: Double labeled: red - Hnf4a; green - laminin.
Structure Level Pattern Image Note
TS19: liver Present Clusters 4N Hepatoblast colonize liver lobes, but form clusters.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory