Gene Expression Data
Assay Details
Assay
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Reference:
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J:51778
Roelen BA, et al., Identification of two distinct functions for TGF-beta in early mouse development. Differentiation. 1998 Nov;64(1):19-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6379856
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Gene symbol:
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Tgfbr2
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Gene name:
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transforming growth factor, beta receptor II
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Antibody:
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anti-TbetaR-II
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Assay notes:
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A tyramide-biotin amplification step was used.
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Results
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Specimen
2B:
embryonic day 4.0
(more )
Specimen
2B:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
embryonic day 4.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS5: trophectoderm |
Present |
|
2B
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Expression is at the plasma membranes of the trophectoderm cells and at the endoplasmatic reticulum close to the nucleus.
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TS5: inner cell mass |
Absent |
|
2B
|
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Specimen
not shown zygote:
embryonic day 0.5
(more )
Note: Zygote stage embryo.
Specimen
not shown zygote:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
|
Age:
|
embryonic day 0.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
Zygote stage embryo.
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Specimen
not shown 2-cell:
embryonic day 1.0
(more )
Note: 2-cell stage embryo.
Specimen
not shown 2-cell:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
optical section
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Fixation:
|
2% Paraformaldehyde
|
Note:
|
2-cell stage embryo.
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Specimen
not shown uncompacted:
embryonic day 3.0
(more )
Note: Uncompacted morula stage embryo.
Specimen
not shown uncompacted:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
Uncompacted morula stage embryo.
|
Specimen
not shown compacted:
embryonic day 3.5
(more )
Note: Compacted morula stage embryo.
Specimen
not shown compacted:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
Compacted morula stage embryo.
|
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