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Gene Expression Data
Assay Details
Assay
Reference: J:168162 Dunning KR, et al., Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol Reprod. 2010 Dec;83(6):909-18
Assay type: RT-PCR
MGI Accession ID: MGI:6379752
Gene symbol: Cpt1b
Gene name: carnitine palmitoyltransferase 1b, muscle
Probe: Cpt1b-pE, Cpt1b-pF
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Data were analyzed relative to a reference or calibrator sample. Results for each PCR were normalized to the calibrator (GC RNA from prepubertal, unstimulated female mice), which was given the arbitrary value of one. Expression was normalized to Rpl19.
Results Image: 2B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
eCG P w 4 TS28: follicle cell of tertiary follicle Present 0.5 µg; total RNA C57BL/6 Female Granulosa cells collected 44 hrs post-equine chorionic gonadotropin (eCG) (immature).
eCG +hCG 4h P w 4 TS28: germinal cell of mature follicle Present 0.5 µg; total RNA C57BL/6 Female Granulosa cells collected 44 hrs post-eCG and 4 hrs post-human chorionic gonadotropin (pre-ovulatory).
eCG +hCG 10h P w 4 TS28: germinal cell of mature follicle Present 0.5 µg; total RNA C57BL/6 Female Granulosa cells collected 44 hrs post-eCG and 10 hrs post-human chorionic gonadotropin (pre-ovulatory).
eCG +hCG 14h P w 4 TS28: germinal cell of ruptured follicle Present 0.5 µg; total RNA C57BL/6 Female Granulosa cells collected 44 hrs post-eCG and 14 hrs post-human chorionic gonadotropin (post-ovulatory).
Notes:
(a) No significant induction of expression was found at any of the time points sampled.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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