Gene Expression Data
Assay Details
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Assay
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Reference:
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J:144178
Couillard M, et al., C-myc as a modulator of renal stem/progenitor cell population. Dev Dyn. 2009 Feb;238(2):405-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6379571
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Gene symbol:
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Six2
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Gene name:
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sine oculis-related homeobox 2
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Antibody:
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anti-Six2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
5F:
embryonic day 12.5; Myctm2Fwa/Myc +
(more  )
Specimen
5F:
(close  )
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Genetic Background:
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involves: 129S/SvEv * 129S6/SvEvTac
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Mutant Allele(s):
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Myctm2Fwa/Myc+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cap mesenchyme |
Present |
Regionally restricted |
5F
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Expression is on the dorsal side of the ureteric bud.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S6/SvEvTac
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Mutant Allele(s):
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Bmp7tm6(cre)Rob/Bmp7+
Myctm2Fwa/Myctm2Fwa
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cap mesenchyme |
Present |
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5G
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Expression in this mutant is the same as in control embryos.
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Specimen
5H:
embryonic day 15.5; Myctm2Fwa/Myc +
(more )
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S6/SvEvTac
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Mutant Allele(s):
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Myctm2Fwa/Myc+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cap mesenchyme |
Present |
Regionally restricted |
5H
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Expression is in multiple layers of condensed cap mesenchyme surrounding ureteric bud tips.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S6/SvEvTac
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Mutant Allele(s):
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Bmp7tm6(cre)Rob/Bmp7+
Myctm2Fwa/Myctm2Fwa
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cap mesenchyme |
Present |
Regionally restricted |
5I
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Expression is in one to two layers of condensed cap mesenchyme surrounding ureteric bud tips and expression was reduced by 30% compared to controls.
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Analysis Tools