Antibody:	anti-Nnat (ab27266)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Two antibodies were described: MGI:6379323 and MGI:5908547; the one used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4A WT Cortex	Expression was predominantly localized in the cell soma and to a lesser level in neuronal processes.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4A AS Cortex	Expression level was comparatively increased in the Angelman Syndrome mice with respect to wild type mice.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		4A WT Hippocampus	Expression was predominantly localized in the cell soma and to a lesser level in neuronal processes.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		4A AS Hippocampus	Expression level was comparatively increased in the Angelman Syndrome mice with respect to wild type mice.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		4B WT Hippocampus Nnat	Expression was detected in Parvalbumin-positive neurons and in other types of neurons.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		4B AS Hippocampus Nnat	Expression level was comparatively higher in Parvalbumin-positive neurons in Angelman Syndrome mice with regard to wild type controls.

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		4B WT Amygdala Nnat	Expression was detected in Parvalbumin-positive neurons and in other types of neurons.

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		4B AS Amygdala Nnat	Expression level was comparatively higher in Parvalbumin-positive neurons in Angelman Syndrome mice with regard to wild type controls.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		S1_WT Cortex

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		S1_AS Cortex	Expression level was comparatively increased in the Angelman Syndrome mice with respect to wild type mice.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		S1_WT Hippocampus

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		S1_AS Hippocampus	Expression level was comparatively increased in the Angelman Syndrome mice with respect to wild type mice.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		S1_WT Hypothalamus

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		S1_AS Hypothalamus	Expression level was comparatively increased in the Angelman Syndrome mice with respect to wild type mice.