Probe:	Six1 probe11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Moderate		1A
TS15: nasal process mesenchyme	Present	Regionally restricted	1A	Expression in mesenchyme underlying olfactory placode.
TS15: future forebrain	Absent		1A

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Strong		1B
TS17: nasal pit	Present	Regionally restricted	1B	Expression is excluded from tip and rim of pit.
TS17: nasal process mesenchyme	Present	Regionally restricted	1B	Expression in mesenchyme underlying olfactory epithelium.
TS17: forebrain	Absent		1B

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present	Widespread	1C
TS19: nasal epithelium	Present	Regionally restricted	1C	Expression in epithelium of vomeronasal groove.
TS19: latero-nasal process mesenchyme	Strong		1C
TS19: head mesenchyme	Present	Regionally restricted	1C	Expression in head mesenchyme near forebrain.
TS19: forebrain	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		1D
TS20: nasal cavity epithelium	Present	Regionally restricted	1D	Weaker expression near orifice of epithelium compared with that in the olfactory epithelium.
TS20: latero-nasal process mesenchyme	Strong		1D
TS20: head mesenchyme	Present	Regionally restricted	1D	Expression in head mesenchyme near forebrain.
TS20: forebrain	Absent		1D

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	1E	Expression in apical compartment of olfactory epithelium, where proliferating sustentacular cells reside. Scattered pattern of expression in basal compartment, at site of proliferating olfactory receptor neuron progenitors. No expression in differentiated olfactory receptor neurons in middle compartment of olfactory epithelium.
TS22: nasal cavity lamina propria	Strong	Regionally restricted	1E	Abundant expression in future lamina propria underlying the olfactory epithelium.
TS22: nasal septum cartilage	Absent		1E	No expression in cartilaginous tissue surrounding the olfactory epithelium, including the future nasal septum.
TS22: brain meninges	Present	Regionally restricted	1E	Expression in meninges covering surface of developing olfactory bulb.
TS22: nasal cavity respiratory epithelium	Weak		1E
TS22: forebrain	Absent		1E
TS22: main olfactory bulb	Absent		1E

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	1F	Expression in apical compartment of olfactory epithelium. Sparce, scattered expression in basal compartment.
TS24: vomeronasal organ epithelium	Present		1F
TS24: lateral nasal gland	Present		1F

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		10B Six1 WT	Authors report similar levels of expression in wild type and Mash1-/- embryos.

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		10B Six1 Mash1-/-	Authors report similar levels of expression in wild type and Mash1-/- embryos.