Dlk1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:194130 Appelbe OK, et al., Conditional deletions refine the embryonic requirement for Dlk1. Mech Dev. 2013 Feb;130(2-3):143-59
Assay type:	Northern blot
MGI Accession ID:	MGI:6377437
Gene symbol:	Dlk1
Gene name:	delta like non-canonical Notch ligand 1

Probe:	Dlk1 probe3
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	Dlk1 is an imprinted gene; it is expressed only from the paternally inherited allele.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2 kb	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
WT	E12.5	TS20: embryo	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT	E12.5	TS20: embryo	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT	E12.5	TS20: embryo	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
Dlk1^cons-pat	E12.5	TS20: embryo	Weak (a)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.
Dlk1^cons-pat	E12.5	TS20: embryo	Weak (a)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.
Dlk1^cons-pat	E12.5	TS20: embryo	Weak (a)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.
WT	E12.5	TS28: placenta	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT	E12.5	TS28: placenta	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT	E12.5	TS28: placenta	Present	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N		Not Specified	The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
Dlk1^cons-pat	E12.5	TS28: placenta	Weak (b)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.
Dlk1^cons-pat	E12.5	TS28: placenta	Weak (b)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.
Dlk1^cons-pat	E12.5	TS28: placenta	Weak (b)	Not Specified; total RNA	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	Dlk1^tm1.2Jvs/Dlk1⁺	Not Specified	Paternally inherited allele.

Notes:
(a) Expression was found to be 7.3% of the wild type level in specimens that inherited the constitutive allele paternally.
(b) Expression was found to be 3.7% of the wild type level in specimens that inherited the constitutive allele paternally.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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