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Gene Expression Data
Assay Details
Assay
Reference: J:194130 Appelbe OK, et al., Conditional deletions refine the embryonic requirement for Dlk1. Mech Dev. 2013 Feb;130(2-3):143-59
Assay type: Northern blot
MGI Accession ID: MGI:6377436
Gene symbol: Dlk1
Gene name: delta like non-canonical Notch ligand 1
Probe: Dlk1 probe3
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Phosphorimaging
Assay notes: Dlk1 is an imprinted gene; it is expressed only from the paternally inherited allele.
Results
Image: 2A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 2 kb Amount Genetic BackgroundMutant Allele(s)Sex Note
WT E12.5 TS20: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT E12.5 TS20: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT E12.5 TS20: embryo Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
Dlk1flox-pat E12.5 TS20: embryo Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
Dlk1flox-pat E12.5 TS20: embryo Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
Dlk1flox-pat E12.5 TS20: embryo Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
WT E12.5 TS28: placenta Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT E12.5 TS28: placenta Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
WT E12.5 TS28: placenta Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Not Specified The genetic background of the specimen was either wild type littermate or one in which the floxed allele was inherited maternally.
Dlk1flox-pat E12.5 TS28: placenta Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
Dlk1flox-pat E12.5 TS28: placenta Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
Dlk1flox-pat E12.5 TS28: placenta Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * FVB/N Dlk1tm1.1Jvs/Dlk1+Not Specified Paternally inherited allele.
Notes:
(a) Expression in the specimen with the paternal floxed allele was not significantly different from wild type, indicating no effect of the loxP insertion.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory