Dab1 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:279463 Zhang B, et al., Alternative Splicing of Disabled-1 Controls Multipolar-to-Bipolar Transition of Migrating Neurons in the Neocortex. Cereb Cortex. 2018 Oct 1;28(10):3457-3467
Assay type:	RT-PCR
MGI Accession ID:	MGI:6377282
Gene symbol:	Dab1
Gene name:	disabled 1

Probe:	Ex6-F2, Ex9-R
Assay notes:	The neocortex was microdissected into upper (U, mainly cortical plate or preplate) and lower halves (L, mainly ventricular/subventricular zone, and intermediate zone) prior to RNA isolation.

Results

Image: 2C Ex6/9

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	246 bp (a)	207 bp (b)	180 bp (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
U	E12.5	TS20: cortical preplate	Absent	Present	Present	Not Specified; total RNA	Not Specified		Not Specified	Microdissected upper cerebral cortex.
L	E12.5	TS20: cerebral cortex intermediate zone	Absent	Present	Present	Not Specified; total RNA	Not Specified		Not Specified	Microdissected lower cerebral cortex.
U	E14.5	TS22: cerebral cortex intermediate zone TS22: cortical plate	Present	Present	Present	Not Specified; total RNA	Not Specified		Not Specified	Microdissected upper cerebral cortex.
L	E14.5	TS22: cerebral cortex subventricular zone TS22: cerebral cortex ventricular layer	Present	Present	Present	Not Specified; total RNA	Not Specified		Not Specified	Microdissected lower cerebral cortex.
U	E18.5	TS26: cortical plate	Present	Present	Present	Not Specified; total RNA	Not Specified		Not Specified	Microdissected upper cerebral cortex.
L	E18.5	TS26: cerebral cortex intermediate zone TS26: cerebral cortex subventricular zone TS26: cerebral cortex ventricular zone	Strong	Absent	Absent	Not Specified; total RNA	Not Specified		Not Specified	Microdissected lower cerebral cortex.

Notes:
(a) This band represents exons 6-9.
(b) This band represents exons 6,8,9.
(c) This band represents exons 6,7,9.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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