Antibody:	Anti-E2F-5 (E19)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: brain	Strong		2A
TS20: 4th ventricle choroid plexus	Strong	Regionally restricted	2A , 2A insert	Strong nuclear expression was detected in the epithelium, but the mesenchyme was largely negative.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		2B insert	Nuclear staining was noted.

Structure	Level	Pattern	Image	Note
TS21: lateral ventricle choroid plexus	Strong	Non-Uniform	2C	Intense nuclear staining was detected in the epithelium, whereas a faint cytoplasmic staining was observed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Strong	Non-Uniform	2D	Intense nuclear staining was detected in the epithelium, whereas a faint cytoplasmic staining was observed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: brain	Strong	Non-Uniform		Staining was observed throughout the brain but was stronger in the periventricular regions.

Structure	Level	Pattern	Image	Note
TS25: 4th ventricle choroid plexus	Weak	Regionally restricted	2E	Staining in the epithelium was markedly reduced at E17.5 and had become predominantly cytoplasmic.

Structure	Level	Pattern	Image	Note
TS25: brain subventricular zone	Present
TS25: brain ventricular layer	Present
TS25: neocortex	Strong	Regionally restricted		Staining of the whisker barrels was intensely positive and nuclear.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Strong	Regionally restricted	3C	Extensive staining was detected throughout the epithelium.

Structure	Level	Pattern	Image	Note
TS25: 4th ventricle choroid plexus	Weak	Regionally restricted	3F	Expression was detected in the epithelium, although it was weaker than at earlier stages.