Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119875
Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6368138
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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Isl1 probe30
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
6G:
embryonic day 10.5
(more  )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6G:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
6G
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Expression in motoneurons.
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Specimen
6H:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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Specimen
6I:
embryonic day 10.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6I:
(close )
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Genetic Background:
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involves: C3H/HeJ * CD-1
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
6I
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Expression not significantly affected.
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| TS17: rhombomere 4 |
Present |
Regionally restricted |
6I
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Slight increase of expression domain in rhombomere 4.
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Analysis Tools