Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119875
Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6368133
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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pGli-3a
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
S2D:
embryonic day 9.5
(more  )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
S2D:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Uniform |
S2D
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Uniform expression at all anterior-posterior levels.
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Specimen
E9.5 Gli2-/- not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Uniform |
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Expression not altered compared to wild type.
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Specimen
S2H:
embryonic day 10.5
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
S2H:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Uniform |
S2H
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Uniform expression at all anterior-posterior levels.
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Specimen
E10.5 Gli2-/- not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Uniform |
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Expression not altered compared to wild type.
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Analysis Tools