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Gene Expression Data
Assay Details
Assay
Reference: J:119875 Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
Assay type: RNA in situ
MGI Accession ID: MGI:6368123
Gene symbol: Nkx6-1
Gene name: NK6 homeobox 1
Probe: cNkx6.1 probe1
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 3K: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3K Expression domain encompasses progenitor domains of V3 and V2 interneurons as well as motoneurons.
Specimen 3L: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: future spinal cord Absent 3L
Specimen 3M: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present 3M Authors report expression was normal.
Specimen 3N: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: future spinal cord Weak Regionally restricted 3N Expression was reduced compared with wildtype.
Specimen 3O: embryonic day 10.5; Gli3Xt-J/Gli3Xt-J, Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present 3O Authors report expression was restored.
Specimen 3P: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present Regionally restricted 3P Expression domain encompasses progenitor domains of V3 and V2 interneurons as well as motoneurons.
Specimen 3Q: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Absent 3Q
Specimen 3R: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present Regionally restricted 3R Expression domain slightly reduced and shifted ventrally.
Specimen 3S: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Weak 3S Expression was reduced compared with wildtype.
Specimen 3T: embryonic day 10.5; Gli3Xt-J/Gli3Xt-J, Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present 3T Authors report expression was restored.
Specimen S1B: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted S1B Authors state expression was disrupted differently in two mutant embryos.
Specimen S1D: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted S1D Authors state expression was disrupted differently in two mutant embryos.
Specimen 4B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: embryo Present 4B
Specimen 4E: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 4E Expression in posterior hindbrain.
TS17: future spinal cord Absent 4E Expression absent from all other locations along then neural tube.
Specimen 4K: embryonic day 10.5 (more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 4K
TS17: rhombomere 7 ventricular layer Present 4K
Specimen 4L: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 4L
TS17: rhombomere 7 mantle layer Present 4L Expression only in mantle layer of neural tube
Specimen 4M: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 4M Gradual ventral shift in expression in hindbrain segments anterior to rhombomere 7.
TS17: rhombomere 1 Ambiguous 4M Very little or no expression in rhombomeres 1, 2 and 3.
TS17: rhombomere 2 Ambiguous 4M Very little or no expression in rhombomeres 1, 2 and 3.
TS17: rhombomere 3 Ambiguous 4M Very little or no expression in rhombomeres 1, 2 and 3.
TS17: rhombomere 4 Present 4M Expression less affected in rhombomere 4 than in neighboring rhombomeres.
Specimen 4N: embryonic day 10.5; Gli3Xt-J/Gli3Xt-J (more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Structure Level Pattern Image Note
TS17: rhombomere 1 Strong Regionally restricted 4N Expression dorsally expanded in rhombomere 1.
TS17: rhombomere 2 Strong 4N Stronger expression in rhombomeres 1, 2 and 3.
TS17: rhombomere 3 Strong 4N Stronger expression in rhombomeres 1, 2 and 3.
TS17: rhombomere Present Regionally restricted 4N Normal levels of expression in the other rhombomeres.
Specimen S3P r2: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 2 Present S3P r2
Specimen S3P r4: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 4 Present S3P r4
Specimen S3P r5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present S3P r5
Specimen S3P r7: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 7 ventricular layer Present S3P r7
Specimen S3Q r2: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 2 Absent S3Q r2
Specimen S3Q r4: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 4 Absent S3Q r4
Specimen S3Q r5: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Absent S3Q r5
Specimen S3Q r7: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Structure Level Pattern Image Note
TS17: rhombomere 7 mantle layer Present S3Q r7 Expression only in mantle layer.
TS17: rhombomere 7 ventricular layer Absent S3Q r7
Specimen S3R r2: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: rhombomere 2 Present S3R r2
Specimen S3R r4: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: rhombomere 4 Present S3R r4 Expression less affected in rhombomere 4 than in neighboring rhombomeres.
Specimen S3R r5: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present S3R r5
Specimen S3R r7: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Structure Level Pattern Image Note
TS17: rhombomere 7 Present S3R r7
Specimen S3S r2: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: rhombomere 2 Strong S3S r2 Stronger expression in rhombomeres 1, 2 and 3.
Specimen S3S r4: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: rhombomere 4 Present S3S r4
Specimen S3S r5: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present S3S r5
Specimen S3S r7: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: rhombomere 7 Present S3S r7
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory