Myf5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153078 Shoji H, et al., Expression pattern and role of Galectin1 during early mouse myogenesis. Dev Growth Differ. 2009 Sep;51(7):607-15
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6363628
Gene symbol:	Myf5
Gene name:	myogenic factor 5

Reporter:	lacZ
Antibody:	anti-B-galactosidase (Sigma)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Results

Specimen 2A top: embryonic day 10.5; Lgals1^tm1Rob/Lgals1⁺, Myf5^tm2Pas/Myf5⁺ (more

)
Note: Double labeled: red - Myf5 reporter; green - Lgals1 (assay MGI:6363622).

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		2A merge top , 2A Myf5 top

Specimen 2A bottom: embryonic day 10.5; Lgals1^tm1Rob/Lgals1⁺, Myf5^tm2Pas/Myf5⁺ (more

)
Note: Double labeled: red - Myf5 reporter; green - Lgals1 (assay MGI:6363622).

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		2A Myf5 bottom , 2A merge bottom

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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