Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56495
Segre JA, et al., Klf4 is a transcription factor required for establishing the barrier function of the skin. Nat Genet. 1999 Aug;22(4):356-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6363544
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Gene symbol:
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Loricrin
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Gene name:
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loricrin cornified envelope precursor protein
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Antibody:
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anti-loricrin
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Detection system:
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Secondary antibody coupled to colloidal gold
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Assay notes:
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Electron microscopy on ultrathin sections. Specimens were fixed in 2% glutaraldehyde, 2% paraformaldehyde, postfixed in 0.5% osmium tetroxide, and were embedded in LX-112 resin.
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Results
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Specimen
4a:
postnatal newborn
(more  )
Specimen
4a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Specimen
4b:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Klf4tm1Efu/Klf4tm1Efu
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: arm skin |
Present |
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4b inset
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Expression is at the inner surface of of cornified envelope in mutant squames.
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| TS27: epidermis granular layer |
Present |
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4b inset
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Expression is at the inner surface of of cornified envelope in mutant squames.
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Analysis Tools