Gene Expression Data
Assay Details
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Assay
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Reference:
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J:25959
Kawano H, et al., Migration of dopaminergic neurons in the embryonic mesencephalon of mice. Brain Res Dev Brain Res. 1995 May 26;86(1-2):101-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6363034
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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anti-NF (160kDa)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The fixative contained 0.5% picric acid. Coronal sections.
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Results
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Specimen
4E:
embryonic day 10.5
(more  )
Specimen
4E:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
4E
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Expression is in tangentially arranged nerve fibers in the basal mesencephalon crossing the ventral midline.
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Specimen
4F:
embryonic day 11.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
4F
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Expression is in tangentially arranged nerve fibers in the ventral half of the ventral mesencephalon.
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Specimen
4G:
embryonic day 13.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
4G
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Expression is in tangentially arranged nerve fibers in the ventral half of the ventral mesencephalon.
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Specimen
4H:
embryonic day 14.5
(more )
Specimen
4H:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
4H
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Expression is in tangentially arranged nerve fibers in the ventral half of the ventral mesencephalon.
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Specimen
5E:
embryonic day 13.5
(more )
Note: Double labeled: brown - Nefm; blue - Th (assay MGI:6363031).
Specimen
5E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Nefm; blue - Th (assay MGI:6363031).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
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5E
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Expression is in tangentially arranged nerve fibers in the ventral half of the ventral mesencephalon.
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Specimen
Not shown E9:
embryonic day 9.5
(more )
Specimen
Not shown E9:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
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Expression is in some neurons in the latero-basal part of the ventral mesencephalon.
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Specimen
Not shown adult:
postnatal adult
(more )
Specimen
Not shown adult:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
Present |
Regionally restricted |
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Expression is in tangentially arranged nerve fibers in the ventral half of the ventral mesencephalon.
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Analysis Tools