Gene Expression Data
Assay Details
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Assay
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Reference:
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J:25959
Kawano H, et al., Migration of dopaminergic neurons in the embryonic mesencephalon of mice. Brain Res Dev Brain Res. 1995 May 26;86(1-2):101-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6363031
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The paraformaldehyde fixative contained 0.5% picric acid. Coronal sections.
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
1A
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Expression is in a few cells in the medial part of the basal mesencephalon.
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Specimen
1B:
embryonic day 11.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
1B
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Expression is in horizontally oriented cells in the medial part of the basal mesencephalon.
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Specimen
1C:
embryonic day 12.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
1C
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Expression is in vertical processes of cells near the ventricular surface and in horizontally oriented cells in the basal mesencephalon.
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Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
1D
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Expression is in vertical processes of cells near the ventricular surface and in horizontally oriented cells in the basal mesencephalon.
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Specimen
1E:
embryonic day 14.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
1E
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Expression is in two lateral groups and has decreased in the vicinity of the ventricular surface.
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Specimen
1F:
embryonic day 16.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: midbrain tegmentum |
Present |
Regionally restricted |
1F
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Expression is in the ventral tegmental area.
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| TS24: midbrain |
Present |
Regionally restricted |
1F
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Expression is in the substantia nigra.
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Specimen
5A:
postnatal day 30
(more )
Note: Double labeled with BrdU (brown).
Specimen
5A:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled with BrdU (brown).
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Specimen
5C:
embryonic day 13.5
(more )
Note: Double labeled: blue - Th; brown - Tnc (assay MGI:6363033).
Specimen
5C:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Th; brown - Tnc (assay MGI:6363033).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
5C
,
5D
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Expression is in vertical processes of cells near the ventricular surface and in horizontally oriented cells in the basal mesencephalon.
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Specimen
5E:
embryonic day 13.5
(more )
Note: Double labeled: blue - Th; brown - Nefm (assay MGI:6363034).
Specimen
5E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Th; brown - Nefm (assay MGI:6363034).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
5E
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Expression is in vertical processes of cells near the ventricular surface and in horizontally oriented cells in the basal mesencephalon.
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Analysis Tools