Gene Expression Data
Assay Details
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Assay
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Reference:
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J:190647
Oshima S, et al., Elevated expression of calcineurin subunits during active mineralization of developing mouse molar teeth. Eur J Oral Sci. 2012 Oct;120(5):386-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6361896
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Gene symbol:
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Ppp3r1
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Gene name:
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protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
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Antibody:
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CnB1 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3C:
embryonic day 18.5
(more  )
Note: Bell stage.
Specimen
3C:
(close  )
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Genetic Background:
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C57BL/6N
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: bell stage tooth |
Strong |
Regionally restricted |
3C
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Strong expression in stratum imtermedium, in pre-ameloblasts, and in pre-odontoblasts. Expression enriched in pre-ameloblasts close to basal lamina.
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Specimen
4G:
postnatal day 3
(more )
Note: Secretory stage.
Specimen
4G:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Secretory stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: molar enamel organ |
Present |
Regionally restricted |
4G
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Diffuse expression in ameloblasts. Strong expression in ameloblasts with an elongated cell shape.
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| TS27: stratum intermedium |
Weak |
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4G
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Weak expression in stratum intermedium.
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Specimen
4H:
postnatal day 10
(more )
Note: Maturation stage.
Specimen
4H:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Maturation stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: molar enamel organ |
Present |
Regionally restricted |
4H
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Strong expression in ruffle-ended ameloblasts. Weak or no expression in smooth-ended ameloblasts. Expression enriched in enamel-forming side of ameloblasts. Expression in perikarya of papillary layer.
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Specimen
5J:
postnatal day 3
(more )
Note: Initiation of dentinogenesis.
Specimen
5J:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Initiation of dentinogenesis.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: molar dental papilla |
Strong |
Regionally restricted |
5J
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Strong expression in odontoblasts. Prominent expression in suprnuclear compartment of odontoblasts close to the predentin layer.
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Specimen
5K:
postnatal day 7
(more )
Note: During dentinogenesis.
Specimen
5K:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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During dentinogenesis.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: molar dental papilla |
Strong |
Regionally restricted |
5K
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Strong expression in odontoblasts. Expression distributed in both regions of the supranuclear compartment.
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Specimen
5L:
postnatal day 14
(more )
Note: Completion of dentinogenesis.
Specimen
5L:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Completion of dentinogenesis.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: molar dental papilla |
Weak |
Regionally restricted |
5L
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Expression substantially lowered in odontoblasts.
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| TS28: molar dental pulp |
Strong |
Regionally restricted |
5L
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Strong expression in subodontoblast layer of the dental pulp.
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Specimen
6G:
postnatal day 14
(more )
Note: Acellular cementum.
Specimen
6G:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Acellular cementum.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cementum |
Moderate |
Regionally restricted |
6G
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Expression in cementoblasts in both cellular and acellular parts of cementum. Moderate expression in acellular cementum.
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Specimen
6H:
postnatal week 4
(more )
Note: Cellular cementum.
Specimen
6H:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal week 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Cellular cementum.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cementum |
Moderate |
Regionally restricted |
6H
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Moderate expression in cementoblasts within the periodontal ligament. Strong expression in cementocytes entrapped in matrix of cellular cementum.
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Specimen
6I:
postnatal day 7
(more )
Note: Bone.
Specimen
6I:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bone.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: jaw skeleton |
Present |
Regionally restricted |
6I
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Expression in osteoblasts lining the surface of the jawbone matrix and in osteocytes entrapped in the bone matrix.
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