Gene Expression Data
Assay Details
Assay
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Reference:
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J:190647
Oshima S, et al., Elevated expression of calcineurin subunits during active mineralization of developing mouse molar teeth. Eur J Oral Sci. 2012 Oct;120(5):386-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6361894
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Gene symbol:
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Ppp3ca
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Gene name:
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protein phosphatase 3, catalytic subunit, alpha isoform
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Antibody:
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CnA alpha antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3A:
embryonic day 18.5
(more )
Note: Bell stage.
Specimen
3A:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bell stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: bell stage tooth |
Strong |
Regionally restricted |
3A
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Strong expression in stratum imtermedium, in pre-ameloblasts, and in pre-odontoblasts. Expression often clustered in perikarya.
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Specimen
4C:
postnatal day 3
(more )
Note: Secretory stage.
Specimen
4C:
(close )
Genetic Background:
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C57BL/6N
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Age:
|
postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Secretory stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: molar enamel organ |
Present |
Regionally restricted |
4C
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Diffuse expression in ameloblasts. Strong expression in ameloblasts with an elongated cell shape. In ameloblasts, expression mainly accumulated in central part of supranuclear compartment.
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Specimen
4D:
postnatal day 10
(more )
Note: Maturation stage.
Specimen
4D:
(close )
Genetic Background:
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C57BL/6N
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Maturation stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: molar enamel organ |
Present |
Regionally restricted |
4D
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Weak expression in ruffle-ended ameloblasts. Weak or no expression in smooth-ended ameloblasts. Expression in perikarya of papillary layer.
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Specimen
5D:
postnatal day 3
(more )
Note: Initiation of dentinogenesis.
Specimen
5D:
(close )
Genetic Background:
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C57BL/6N
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
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Initiation of dentinogenesis.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: molar dental papilla |
Strong |
Regionally restricted |
5D
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Strong expression in odontoblasts. Prominent expression in suprnuclear compartment of odontoblasts close to the predentin layer.
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Specimen
5E:
postnatal day 7
(more )
Note: During dentinogenesis.
Specimen
5E:
(close )
Genetic Background:
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C57BL/6N
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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During dentinogenesis.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: molar dental papilla |
Strong |
Regionally restricted |
5E
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Strong expression in odontoblasts. Ecpression enriched in central part of supranuclear compartment of odontoblasts.
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Specimen
5F:
postnatal day 14
(more )
Note: Completion of dentinogenesis.
Specimen
5F:
(close )
Genetic Background:
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C57BL/6N
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Completion of dentinogenesis.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: molar dental papilla |
Weak |
Regionally restricted |
5F
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Expression substantially lowered in odontoblasts.
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Specimen
6A:
postnatal day 14
(more )
Note: Acellular cementum.
Specimen
6A:
(close )
Genetic Background:
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C57BL/6N
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Age:
|
postnatal day 14
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Acellular cementum.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cementum |
Moderate |
Regionally restricted |
6A
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Expression in cementoblasts in both cellular and acellular parts of cementum. Moderate expression in acellular cementum.
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Specimen
6B:
postnatal week 4
(more )
Note: Cellular cementum.
Specimen
6B:
(close )
Genetic Background:
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C57BL/6N
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Age:
|
postnatal week 4
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Cellular cementum.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cementum |
Strong |
Regionally restricted |
6B
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Strong expression in cellular cementum. Strong expression in cementocytes entrapped in matrix of cellular cementum.
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Specimen
6C:
postnatal day 7
(more )
Note: Bone.
Specimen
6C:
(close )
Genetic Background:
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C57BL/6N
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Age:
|
postnatal day 7
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bone.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: jaw skeleton |
Present |
Regionally restricted |
6C
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Expression in osteoblasts lining the surface of the jawbone matrix and in osteocytes entrapped in the bone matrix.
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