Tg(Aldh1l1-RHO/ADRA1,-lacZ)B36-3Mik
Transgene Detail
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| Symbol: |
Tg(Aldh1l1-RHO/ADRA1,-lacZ)B36-3Mik |
| Name: |
transgene insertion B36-3, M I Kotlikoff |
| MGI ID: |
MGI:6361524 |
| Synonyms: |
Aldh1L1-Opto alpha1AR-IRES-lacZ, B36-Aldh1l1BAC-Optoa1AR_IRES_lacZ |
| Transgene: |
Tg(Aldh1l1-RHO/ADRA1,-lacZ)B36-3Mik Location: unknown
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| Alliance: |
Tg(Aldh1l1-RHO/ADRA1,-lacZ)B36-3Mik page
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| Strain of Origin: |
(C57BL/6J x SJL/J)F2
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The ~228 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-7M9 was obtained; containing the protein coding region of the Aldh1l1 as well as ~170 kbp of telomeric flanking sequences (including the complete Slc41a3 locus and a portion of the Iqsec1 locus). Using homologous recombination/BAC recombineering, a 6170 bp Opto alpha1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Aldh1l1 gene. No other loci on the BAC were altered. The 11.6 kbp BAC vector contained a chloramphenicol selection cassette. The fusion protein is a Gt-coupled bovine rhodopsin sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gq-coupled human ADRA1A.
(J:314125)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858 |
| All: |
1 reference(s) |
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Analysis Tools
