Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik
Name:	transgene insertion B22-1, M I Kotlikoff
MGI ID:	MGI:6361521
Synonyms:	B22-LckBAC-Optoa1AR_IRES_lacZ, Lck-Opto alpha 1AR-IRES-lacZ
Transgene:	Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik  Location: unknown
Alliance:	Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik page

Strain of Origin:

FVB/N X B6(Cg)-Tyrc-2J/J

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The ~150 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP24-159E19 contains the entire lymphocyte protein tyrosine kinase locus as well as ~63 kbp of centromeric flanking sequences (including the complete Task3 and Hdac1 loci) and ~77 kbp of telomeric flanking sequences (including the complete Fam167b, Eif3i, Dcdc2b, Iqcc and Ccdc28b loci, as well as a portion of the Txlna locus). Using homologous recombination/BAC recombineering, a 6170 bp Optoalpha1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Lck gene. No other loci on the BAC were altered. The 13.4 kbp BAC vector contained a chloramphenicol selection cassette. The fusion protein is a Gt-coupled bovine rhodopsin (RHO) sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gq-coupled human alpha 1a-adrenergic receptor. (J:314125)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
All:	1 reference(s)