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Gene Expression Data
Assay Details
Assay
Reference: J:276416 Zhuang M, et al., The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression. Nucleic Acids Res. 2019 May 21;47(9):4765-4777
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361470
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Antibody: anti-Robo3.1 (rabbit)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Assay notes: Another antibody, MGI:6361436, gave the generated identical immunostaining patterns.
Results
Specimen 1C left: embryonic day 11.5; Gli2tm2.1Alj/Gli2+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1C lower left , 1C upper left Expressed only in precrossing and midline-crossing commissural axons, but not post-crossing axons.
Specimen 1C right: embryonic day 11.5; Gli2tm2.1Alj/Gli2tm2.1Alj (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1C upper right , 1C lower right Expression was elevated in crossing axons andmaintained in post-crossing axons in floor plate-deficient spinal cord, compared with heterozygous control littermates.
Specimen S1B: embryonic day 11.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Atoh1-cre)1Bfri/0 (more )
Note: Conditional mutant. Double labeled: red - Robo3; green - Cre reporter.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S1B Expressed only in precrossing and midline-crossing commissural axons, but not post-crossing axons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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