Gt(ROSA)26Sortm3(CAG-EYFP*,-tdTomato*,-cre)Ben
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm3(CAG-EYFP*,-tdTomato*,-cre)Ben |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Rui Benedito |
| MGI ID: |
MGI:6361135 |
| Synonyms: |
Gt(ROSA)26Sor-iSuRe-Cre |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm3(CAG-EYFP*,-tdTomato*,-cre)Ben page
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| Allele Type: |
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Targeted (Conditional ready, Recombinase, Reporter) |
| Mutation: |
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Insertion
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Gt(ROSA)26Sortm3(CAG-EYFP*,-tdTomato*,-cre)Ben expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| chicken |
CAG |
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Mutation details: The allele contains four chicken beta-globin HS4 insulator sequences (two on each ends) flanking the ubiquitous and strong CAG promoter driving LoxP-flanked (floxed) DNA sequence which has the N-PhiM reporter gene encoding a noncytotoxicand non-fluorescent mutated PhiYFP protein with a nuclear localization signal and a transcription stop signal followed by bright membrane-localized fluorescent reporter (tdTomato*) and cre gene separated by 2A cleavage site (T2A; mediates ribosomal skipping), then a WPRE sequence, an SV40 polyA signal. A Frt flanked PGK-neo cassette followed those sequences. Despite lack of recombination/deletion of the floxed cassette by Cre, the modified dtTomato and Cre protein are constitutively active and permanently expressed.
(J:278452)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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CAG
(chicken)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gt(ROSA)26Sor Mutation: |
1052 strains or lines available
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Leakiness of the promoter in the male germline precludes it from being used as originally intended.
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| Original: |
J:278452 Fernandez-Chacon M, et al., iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications. Nat Commun. 2019 May 22;10(1):2262 |
| All: |
6 reference(s) |
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Analysis Tools
