Gene Expression Data
Assay Details
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Assay
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Reference:
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J:108393
Chung AC, et al., Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer. Dev Biol. 2006 May 1;293(1):13-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6360882
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Gene symbol:
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Pax5
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Gene name:
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paired box 5
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Probe:
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5sg8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 8.5
(more  )
Note: 6 somites
Specimen
6A:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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6 somites
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nr6a1tm1Coo/Nr6a1tm1Coo
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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6 somites
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Specimen
6C:
embryonic day 9.5
(more )
Note: 30 somites
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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30 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: midbrain-hindbrain junction |
Present |
Regionally restricted |
6C
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Expression domains refined to highly ordered pattern in midbrain-hindbrain boundary.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nr6a1tm1Coo/Nr6a1tm1Coo
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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13 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural ectoderm |
Weak |
Regionally restricted |
6D
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Weak expression in a ridge of neuroectoderm, while medial expression was lost.
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Analysis Tools
