Antibody:	anti-nidogen
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS20: basement membrane	Present	Regionally restricted	5A	Expression is in the basement membrane of the ureter buds and in the basement membrane of the capillaries in the kidney.
TS20: epithelial layer of ureter	Present	Regionally restricted	5A	Expression is in the basement membrane of the ureter buds.
TS20: mesenchyme of metanephros	Present	Regionally restricted	5A	Expression is punctate in in the interphase between the condensing mesenchyme and the loose mesenchyme and in the loose mesenchyme itself.
TS20: blood vessel endothelium	Present	Regionally restricted	5A	Expression is in the basement membrane of the capillaries in the kidney.

Structure	Level	Pattern	Image	Note
TS22: blood vessel endothelium	Present	Regionally restricted	6A	Expression is in the basement membranes of blood vessels.
TS22: basement membrane	Present	Regionally restricted	6A	Expression is in the basement membranes.
TS22: collecting duct	Present	Regionally restricted	6A	Expression is in the basement membranes.
TS22: renal tubule	Present	Regionally restricted	6A	Expression is in the basement membranes.
TS22: s-shaped body	Present	Regionally restricted	6A	Expression is in the basement membranes.
TS22: glomerular basement membrane of maturing renal corpuscle	Present		6A
TS22: mesenchyme of metanephros	Present	Regionally restricted	6A	Expression is uneven in the loose mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present	Regionally restricted	6B	Expression is in the basement membranes.
TS22: mesenchyme of metanephros	Strong	Regionally restricted	6B	Expression is in mesenchyme surrounding the epithelium of the S-shaped body and there is little expression in the stroma close to the stalk of the ureter.
TS22: basement membrane	Present	Regionally restricted	6B	Expression is in basement membranes of tubular and glomerular structures and in the basement membranes of blood vessel endothelium of the kidney.
TS22: blood vessel endothelium	Present	Regionally restricted	6B	Expression is in the basement membranes.

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present	Regionally restricted	7A	Expression is in the basement membranes.
TS22: trachea epithelium	Present	Regionally restricted	7A	Expression is in the basement membranes.
TS22: blood vessel endothelium	Present	Regionally restricted	7A	Expression is in the basement membranes of the endothelium in the lung.
TS22: lung vascular element	Present	Regionally restricted	7A	Expression is in the basement membranes of the endothelium in the lung.
TS22: basement membrane	Present	Regionally restricted	7A	Expression is in the basement membranes of the tracheal epithelium and in blood vessel endothelium in the lung.
TS22: lung mesenchyme	Absent		7A