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Gene Expression Data
Assay Details
Assay
Reference: J:203015 Panayiotou E, et al., Pax6 is expressed in subsets of V0 and V2 interneurons in the ventral spinal cord in mice. Gene Expr Patterns. 2013 Dec;13(8):328-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6360662
Gene symbol: Pax6
Gene name: paired box 6
Antibody: Anti-Pax6
Assay notes: Two Pax6 antibodies were described MGI:6356544 and MGI:6356545; either could have been used in this assay.
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1A Expression was detected in the ventricular zone.
Specimen 1B: embryonic day 11.5 (more )
Note: Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 1B
Specimen 1C: embryonic day 12.5 (more )
Note: Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS20: spinal cord ventricular layer Present 1C
TS20: spinal cord Present Regionally restricted 1C Expression was detected in a small group of ventrally migrating cells (most likely interneurons).
Specimen 1D: embryonic day 14.5 (more )
Note: Double labeled: green - Pax6; red - BrdU. BrdU was injected at E10.5. Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 1D Expression was detected in ventral interneurons.
Specimen 1E: embryonic day 14.5 (more )
Note: Double labeled: green - Pax6; red - BrdU. BrdU was injected at E11.5. Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 1E Expression was detected in ventral interneurons. The interneurons are mostly specified at E11.5.
Specimen 1F: embryonic day 14.5 (more )
Note: Double labeled: green - Pax6; red - BrdU. BrdU was injected at E12.5. Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 1F Expression was detected in ventral interneurons.
Specimen 5A: embryonic day 14.5 (more )
Note: Triple labeled: green - Pax6; red - Gata3 (assay MGI:6360727); blue - Vsx2 (assay MGI:6360726). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5D , 5A Expression was detected in interneurons. There was some co-expression with Gata3 positive V2b interneurons. There was no co-expression with Vsx2 positive V2a interneurons.
Specimen 5E: embryonic day 14.5; Psen1tm1Pcw/Psen1tm1Pcw (more )
Note: Triple labeled: green - Pax6; red - Gata3 (assay MGI:6360727); blue - Vsx2 (assay MGI:6360726). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5E , 5H In the mutant, the Pax6 positive interneurons were mostly co-expressing Vsx2. There were no Pax6-positive/Gata3-positive cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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