Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:106405
Mackay DR, et al., The mouse Ovol2 gene is required for cranial neural tube development. Dev Biol. 2006 Mar 1;291(1):38-52
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6360630
|
Gene symbol:
|
Wnt6
|
Gene name:
|
wingless-type MMTV integration site family, member 6
|
Probe:
|
Wnt6 cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
6C +/-:
embryonic day 8.5; Ovol2tm1Xdai/Ovol2 +
(more )
Specimen
6C +/-:
(close )
Genetic Background:
|
either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
|
Mutant Allele(s):
|
Ovol2tm1Xdai/Ovol2+
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6C -/-:
(close )
Genetic Background:
|
either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
|
Mutant Allele(s):
|
Ovol2tm1Xdai/Ovol2tm1Xdai
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: surface ectoderm |
Present |
Regionally restricted |
6C -/-
|
Expression in presumptive surface ectoderm juxtaposing dorsal border of neural plate, but ratio of cranial surface ectoderm-to-neuroectoderm reduced two-fold in mutants compared to wild type.
|
|