Probe:	Sox9 cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Weak	Diffuse	1A	A weak diffuse band of expression was noted on the lateral side of the genital ridge in both sexes.
TS17: genitourinary system	Strong	Regionally restricted	1A	The tissue between the genital ridges was strongly expressing Sox9.
TS17: mesonephros	Absent		1A
TS17: embryo mesenchyme	Present	Regionally restricted	1A	Expression was detected in the tissue lying between the mesonephroi, which surrounds the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: skeletal system	Present		1B
TS19: urogenital ridge	Present		1B
TS19: mesonephros	Absent		1B
TS19: embryo mesenchyme	Present	Regionally restricted	1B	Expression was detected in the tissue lying between the mesonephroi, which surrounds the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: skeletal system	Present		1C
TS19: mesonephros	Absent		1C
TS19: urogenital ridge	Absent		1C
TS19: embryo mesenchyme	Present	Regionally restricted	1C	Expression was detected in the tissue lying between the mesonephroi, which surrounds the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Present		1D male left
TS20: primary sex cord	Present		1D male left
TS20: testis	Present		1D male left
TS20: mesonephros	Present	Regionally restricted	1D male left	Expression was detected at the cranial end of the mesonephros.

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Absent		1D female right
TS20: paramesonephric duct	Present		1D female right
TS20: mesonephros	Present	Regionally restricted	1D female right	Expression was detected at the cranial end of the mesonephros.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		1E male left
TS21: testis	Present		1E male left
TS21: male reproductive system	Present	Regionally restricted	1E male left	Expression was detected in the cells surrounding the male Mullerian duct.

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Absent		1E female right

Structure	Level	Pattern	Image	Note
TS25: epididymis	Present		1F male left
TS25: seminiferous cord	Present		1F male left
TS25: testis	Present		1F male left

Structure	Level	Pattern	Image	Note
TS25: ovary	Absent		1F female right

Structure	Level	Pattern	Image	Note
TS20: ureteric tip	Present		6A	Expression was detected in the branching ureteric bud of both sexes.
TS20: nephrogenic interstitium	Absent		6A
TS20: connective tissue	Present	Regionally restricted	6A	Expression was detected in the cells surrounding the presumptive ureter.