Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6358677
|
|
Gene symbol:
|
Epha7
|
|
Gene name:
|
Eph receptor A7
|
|
Probe:
|
Epha7 cDNA3
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
5C:
embryonic day 18.5
(more  )
Specimen
5C:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Non-Uniform |
5C
|
Expression was detected throughout the neocortex but at much lower levels in intermediate parts of the neocortex (somatosensory area).
|
Specimen
5C':
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx2tm1Pgr/Emx2tm1Pgr
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
5C'
|
In Emx2 mutants, the domain of lower expression was reduced in extent and shifted caudally.
|
Specimen
5D:
embryonic day 18.5
(more )
Specimen
5D:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Non-Uniform |
5D
|
Expression was detected throughout the neocortex but at much lower levels in intermediate parts of the neocortex (somatosensory area).
|
Specimen
5D':
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
5D'
|
In Pax6 mutants a domain of lower expression was not evident, but a small domain of reduced expression was apparent and restricted to a more rostral position.
|
Specimen
7E:
embryonic day 18.5
(more )
Specimen
7E:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Weak |
Regionally restricted |
7E
|
Low expression was centered on the somatosensory area.
|
Specimen
7E':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx1tm1Jlr/Emx1tm1Jlr
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Weak |
Regionally restricted |
7E'
|
Expression in Emx1 mutants was very similar to wild type.
|
Specimen
9D:
embryonic day 18.5; Emx1tm1Jlr/Emx1 +
(more )
Specimen
9D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx1tm1Jlr/Emx1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Weak |
Regionally restricted |
9D
|
Low expression was centered on the somatosensory area.
|
Specimen
9D':
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx1tm1Jlr/Emx1tm1Jlr
Emx2tm1Pgr/Emx2tm1Pgr
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Weak |
Regionally restricted |
9D'
|
Expression was shifted caudally in the Emx double mutants.
|
Specimen
9D":
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx2tm1Pgr/Emx2tm1Pgr
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Weak |
Regionally restricted |
9D"
|
Expression was shifted caudally in the Emx2 mutants.
|
|
Analysis Tools