Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358675
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Gene symbol:
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Ngfr
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Gene name:
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nerve growth factor receptor (TNFR superfamily, member 16)
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Probe:
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p75 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4C:
embryonic day 18.5
(more  )
Specimen
4C:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cortical subplate |
Present |
Regionally restricted |
4C
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Expression was detected in subplate and layer 6 of caudal neocortex and tapers off sharply to nondetectable levels rostrally.
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| TS26: neocortex |
Present |
Regionally restricted |
4C
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Expression was detected in subplate and layer 6 of caudal neocortex and tapers off sharply to nondetectable levels rostrally.
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Specimen
4C':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cortical subplate |
Present |
Regionally restricted |
4C'
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In Emx2 mutants, the expression domain is contracted and shifted caudally.
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| TS26: neocortex |
Present |
Regionally restricted |
4C'
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In Emx2 mutants, the expression domain is contracted and shifted caudally.
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Specimen
4D:
embryonic day 18.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cortical subplate |
Present |
Regionally restricted |
4D
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Expression was detected in subplate and layer 6 of caudal neocortex and tapers off sharply to nondetectable levels rostrally.
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| TS26: neocortex |
Present |
Regionally restricted |
4D
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Expression was detected in subplate and layer 6 of caudal neocortex and tapers off sharply to nondetectable levels rostrally.
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Specimen
4D':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
4D'
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In Pax6 mutants, the domain of expression expands rostrally to cover the entire rostrocaudal axis of the neocortex.
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Specimen
7C:
embryonic day 18.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
7C
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Expression was detected in the deep layers in roughly the caudal half of the neocortex.
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Specimen
7C':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
7C'
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Expression in Emx1 mutants was very similar to wild type.
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Specimen
9B:
embryonic day 18.5; Emx1tm1Jlr/Emx1 +
(more )
Specimen
9B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9B
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Expression was detected in the deep layers in the caudal half of the neocortex.
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Specimen
9B':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9B'
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The expression domain constricts caudally in Emx double mutants.
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Specimen
9B":
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9B"
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The expression domain constricts caudally in Emx2 mutants.
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Analysis Tools