Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358674
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Gene symbol:
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Rorb
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Gene name:
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RAR-related orphan receptor beta
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Probe:
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RZR beta cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4A:
embryonic day 18.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Strong |
Graded |
4A
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Graded expression was detected in layer 4 extending across most of the rostrocaudal extent of the neocortex; expression is strong rostrally and declines to very low levels near the extreme caudal pole of the neocortex. Layer 5 is also differentially expressed, with high expression restricted to the rostral pole and a rapid decline to very low levels at the midpoint of the neocortex. Expression was very low or nondetectable in the caudal half of the neocortex.
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Specimen
4A':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Strong |
Regionally restricted |
4A'
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In Emx2 mutants, expression in layer 4 was detected across virtually the entire neocortex. Layer 5 expression was expanded throughout the intermediate parts of the neocortex and well into caudal neocortex.
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Specimen
4B:
embryonic day 18.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Strong |
Graded |
4B
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Graded expression was detected in layer 4 extending across most of the rostrocaudal extent of the neocortex; expression is strong rostrally and declines to very low levels near the extreme caudal pole of the neocortex. Layer 5 is also differentially expressed, with high expression restricted to the rostral pole and a rapid decline to very low levels at the midpoint of the neocortex. Expression was very low or nondetectable in the caudal half of the neocortex.
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Specimen
4B':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
4B'
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In Pax6 mutants, expression constricted rostrally, and there was virtually no expression detected in any layers in the caudal half of the neocortex.
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Specimen
7B:
embryonic day 18.5
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Graded |
7B
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Expression was detected in two distinct high rostral to low caudal gradients across the neocortex; a gradient in superficial layers that extends farther caudally than a gradient within the deeper layers.
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Specimen
7B':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Graded |
7B'
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Expression in Emx1 mutants was very similar to wild type.
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Analysis Tools