Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358673
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Gene symbol:
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Id2
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Gene name:
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inhibitor of DNA binding 2
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Probe:
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Id2 cDNA6
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 18.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3A
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Broad expression was detected across the neocortex. Strong expression was in layer 5 in intermediate and caudal parts of the neocortex, but was weak in rostral parts. The strong to weak expression occurs abruptly at a position that corresponds to the boundary between motor and somatosensory areas. Strong expression was also detected rostrally in layers 2/3, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable.
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Specimen
3A':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3A'
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In Emx2 mutant, the boundary between strong and weak layer 5 expression shifted caudally. Also, the rostral domain of strong expression in layers 2/3 expanded caudally. Only the extreme caudal pole of the cortical hemisphere had a low level of expression.
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Specimen
3B:
embryonic day 18.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3B
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Broad expression was detected across the neocortex. Strong expression was in layer 5 in intermediate and caudal parts of the neocortex, but was weak in rostral parts. The strong to weak expression occurs abruptly at a position that corresponds to the boundary between motor and somatosensory areas. Strong expression was also detected rostrally in layers 2/3, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable.
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Specimen
3B':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3B'
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In Pax6 mutant, the boundary between strong and weak layer 5 expression is shifted rostrally. The domain of strong expression in layers 2/3 was absent throughout the neocortex.
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Specimen
7A:
embryonic day 18.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Graded |
7A
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Graded expression was detected in superficial layers rostrally, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable. Graded low to high expression was noted in layer 5 between motor and somatosensory areas.
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Specimen
7A':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Graded |
7A'
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Expression in Emx1 mutants was very similar to wild type.
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Analysis Tools