Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358672
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Gene symbol:
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Cdh6
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Gene name:
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cadherin 6
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Probe:
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Cad6 cDNA3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3C:
embryonic day 18.5
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3C
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Expression was prominent in dorsal and lateral parts of the neocortex in somatosensory and auditory areas. A domain of low expression was noted, especially in layers 4 and 5, in extreme rostral neocortex.
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Specimen
3C':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3C'
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In Emx2 mutants, the rostral domain of low expression was greatly expanded and covers the rostral half of the neocortex.
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Specimen
3D:
embryonic day 18.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3D
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Expression was prominent in dorsal and lateral parts of the neocortex in somatosensory and auditory areas. A domain of low expression was noted, especially in layers 4 and 5, in extreme rostral neocortex.
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Specimen
3D':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
3D'
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In Pax6 mutants, the rostral domain is essentially absent, and high levels of expression continue to the rostral pole of the neocortex.
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Specimen
9A:
embryonic day 18.5; Emx1tm1Jlr/Emx1 +
(more )
Specimen
9A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9A
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Expression was detected in the neocortex with a low expression domain in far rostral neocortex.
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Specimen
9A':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9A'
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In the double mutant, the domain of low expression expands and shifts caudally (similar to the Emx2 mutant).
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Specimen
9A":
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
9A"
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In the Emx2 mutant, the domain of low expression expands and shifts caudally.
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Analysis Tools