Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78745
Bishop KM, et al., Distinct actions of Emx1, Emx2, and Pax6 in regulating the specification of areas in the developing neocortex. J Neurosci. 2002 Sep 1;22(17):7627-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358652
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Gene symbol:
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Cdh8
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Gene name:
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cadherin 8
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Probe:
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Cad8 cDNA3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2A':
embryonic day 18.5
(more  )
Specimen
2A':
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
2A'
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Broad expression was detected across the neocortex within layer 5; however, throughout rostral neocortex expression was also high in layers 2/3.
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Specimen
2B':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
2B'
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In Emx2 mutants, the superficial rostral expression domain is expanded, and its caudal boundary is shifted caudally. The expression pattern in the somatosensory area also shifts caudally and is found within caudal neocortex. The visual area expression domain appears to be absent.
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Specimen
2C':
embryonic day 18.5
(more )
Specimen
2C':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
2C'
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Broad expression was detected across the neocortex within layer 5; however, throughout rostral neocortex expression was also high in layers 2/3.
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Specimen
2D':
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
2D'
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In Pax6 mutants, the superficial rostral expression domain is absent.
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Specimen
6A':
embryonic day 18.5
(more )
Specimen
6A':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
Regionally restricted |
6A'
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Broad expression was detected across the neocortex within layer 5; however, throughout rostral neocortex expression was also high in layers 2/3.
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Specimen
6B':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx1tm1Jlr/Emx1tm1Jlr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
Present |
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6B'
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The expression pattern in the Emx1 mutant was similar to wild type.
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Analysis Tools