Id4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:27790 Riechmann V, et al., Mutually exclusive expression of two dominant-negative helix-loop-helix (dnHLH) genes, ld4 and ld3, in the developing brain of the mouse suggests distinct regulatory roles of these dnHLH proteins during cellular proliferation and differentiation of the nrvous system. Cell Growth Differ. 1995 Jul;6(7):837-43
Assay type:	RNA in situ
MGI Accession ID:	MGI:6356697
Gene symbol:	Id4
Gene name:	inhibitor of DNA binding 4

Probe:	Id4 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

Specimen 1B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		1B
TS20: spinal cord	Present		1B
TS20: hindbrain	Strong	Regionally restricted	1B	Expressed in the senotonengic nuclear complex.
TS20: hypothalamus	Present	Regionally restricted	1B	Expressed in the postoptic area.
TS20: telencephalon	Present	Regionally restricted	1B	Expressed in the frontal and parietal cortex.
TS20: cerebellum primordium	Absent		1B
TS20: midbrain	Absent		1B
TS20: pons	Absent		1B

Specimen 2B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2B	Expressed in the frontal and parietal cortex.
TS20: medulla oblongata	Present		2B
TS20: optic stalk	Present		2B
TS20: preoptic area	Present		2B
TS20: superior cerebellar peduncle	Present		2B
TS20: hypothalamus	Present	Regionally restricted	2B	Expressed in the supraoptic area.
TS20: hindbrain	Present	Regionally restricted	2B	Expressed in the pontine flexure and pyramidal tract.
TS20: cerebellum primordium	Absent		2B
TS20: midbrain	Absent		2B

Specimen 3B: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		3B
TS22: telencephalon ventricular layer	Present		3B
TS22: trigeminal ganglion	Present		3B
TS22: hindbrain	Strong	Regionally restricted	3B	Expressed in the senotonengic nuclear complex.
TS22: telencephalon	Present	Regionally restricted	3B	Expressed in the frontal and parietal cortex.
TS22: midbrain	Strong		3B
TS22: preoptic area	Strong		3B
TS22: spinal cord	Strong		3B

Specimen 4A/C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong		4A/C
TS22: hindbrain	Strong	Regionally restricted	4A/C	Expressed in the senotonengic nuclear complex.
TS22: telencephalon	Present	Regionally restricted	4A/C	Expressed in the frontal and parietal cortex.
TS22: midbrain	Strong		4A/C
TS22: cerebellum	Weak		4A/C

Specimen 4B/D: embryonic day 14.5 (more

)

Structure	Level	Image
TS22: telencephalon ventricular layer	Present	4B/D
TS22: trigeminal ganglion	Present	4B/D
TS22: preoptic area	Strong	4B/D

Specimen 5B: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Weak		5B
TS25: telencephalon ventricular layer	Weak		5B

Specimen not shown: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo	Trace			Not detected. Transcripts were rare and probably present in only a few cells scattered throughout the embryo.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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