Probe:	Id3 cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Strong		1D
TS20: bulbar ridge	Strong		1D
TS20: pons	Strong		1D
TS20: telencephalon ventricular layer	Strong	Regionally restricted	1D	Expressed in the ventricular zone of the IV ventricle, including the posterior choroid plexus.
TS20: hindlimb	Present		1D
TS20: remnant of Rathke's pouch	Present		1D
TS20: thalamus	Present	Regionally restricted	1D	Expressed in dorsal thalamus.
TS20: midbrain roof plate	Weak	Regionally restricted	1D	Expressed in tectum.
TS20: telencephalon	Present	Regionally restricted	1D	Expressed in the hindwall, an area between the panietal cortex and the dorsal subpallial sulcus.
TS20: diaphragm	Present		1D
TS20: lung	Present		1D
TS20: mandible primordium	Present		1D
TS20: vertebral cartilage condensation	Present		1D
TS20: nasal septum mesenchyme	Present	Regionally restricted	1D	Expressed in nasal cartilage.

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Strong	Regionally restricted	2C	Expressed in the ventricular zone of the IV ventricle, including the posterior choroid plexus.
TS20: thalamus	Present	Regionally restricted	2C	Expressed in dorsal thalamus.
TS20: telencephalon	Present	Regionally restricted	2C	Expressed in the hindwall, an area between the panietal cortex and the dorsal subpallial sulcus.
TS20: optic stalk	Absent		2C
TS20: superior cerebellar peduncle	Absent		2C

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Regionally restricted	3D	Expressed in the ventricular zone of the IV ventricle.
TS22: telencephalon	Present	Regionally restricted	3D	Expressed in the hindwall.
TS22: dorsal root ganglion	Present	Regionally restricted	3D	Expressed in the outer layer.
TS22: trigeminal ganglion	Present	Regionally restricted	3D	Expressed in the outer layer.
TS22: inner ear	Present		3D	otic vesicle
TS22: Meckel's cartilage	Present		3D
TS22: cranium	Present		3D
TS22: diaphragm	Present		3D
TS22: forelimb interdigital region	Present		3D
TS22: hindlimb interdigital region	Present		3D
TS22: nasal septum cartilage	Present		3D
TS22: vertebral cartilage condensation	Present		3D
TS22: lung epithelium	Present		3D
TS22: stomach	Present		3D
TS22: heart	Absent		3D
TS22: liver	Absent		3D
TS22: metencephalon	Absent		3D
TS22: midbrain	Absent		3D
TS22: preoptic area	Absent		3D
TS22: spinal cord	Absent		3D

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Regionally restricted	4E	Expressed in the ventricular zone of the IV ventricle, including the posterior choroid plexus.
TS22: telencephalon	Present	Regionally restricted	4E	Expressed in the hindwall.
TS22: inner ear	Present		4E	otic vesicle

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	4F	Expressed in the hindwall.
TS22: telencephalon ventricular layer	Present	Regionally restricted	4F	Expressed in the ventricular zone of the IV ventricle.
TS22: cranium	Present		4F

Structure	Level	Pattern	Image	Note
TS25: gut	Strong		5D
TS25: lung epithelium	Strong		5D
TS25: metanephros	Strong		5D
TS25: cochlea	Present		5D
TS25: diaphragm	Present		5D
TS25: olfactory epithelium	Present		5D
TS25: orbito-sphenoid	Present		5D
TS25: semicircular canal	Present		5D
TS25: temporal bone petrous part	Present		5D
TS25: head bone	Present	Regionally restricted	5D	Expressed in the occipital bone and other cartilage primordia.
TS25: forelimb bone	Present	Regionally restricted	5D	Most prominent in the ossification structures within cartilage primordia.
TS25: hindlimb bone	Present	Regionally restricted	5D	Most prominent in the ossification structures within cartilage primordia.
TS25: rib	Present	Regionally restricted	5D	Most prominent in the ossification structures within cartilage primordia.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present