Probe:	mFz8 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		1A	Expression in distal visceral endoderm at tip of the egg cylinder.

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present	Regionally restricted	1B	Asymmetric expression in visceral endoderm shifts proximally, toward the anterior.

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		1C	Expresion localizes to anterior visceral endoderm at E6.0 prior to primitive streak formation.

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present		1D	Expression restricted to endoderm layer.

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		1E	Expression restricted to endoderm layer.

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Present		2A
TS9: primitive streak	Present	Regionally restricted	2A	Second domain of expression at anterior end of primitive streak. Authors state this represents anterior mesendoderm.

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		2B
TS10: primitive streak	Present	Regionally restricted	2B	Expression extends rostrally from streak.

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		2C
TS10: primitive streak	Present	Regionally restricted	2C	Expression in anterior streak cells.
TS10: mesendoderm	Present	Regionally restricted	2C	Expression in cells delaminating to form the anterior mesendoderm, which intercalate with cells in anterior visceral endoderm.

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		2D	Expression in ectoderm and endoderm layers.
TS10: mesendoderm	Present		2D	Expression in ectoderm and endoderm layers.
TS10: primitive streak	Present		2D	Expression in ectoderm and endoderm layers.

Structure	Level	Pattern	Image	Note
TS10: mesendoderm	Present	Regionally restricted	2E	Authors state that anterior mesendoderm is specifically marked by Fzd8 expression.

Structure	Level	Pattern	Image	Note
TS10: mesendoderm	Present	Regionally restricted	2F	Expression in more caudal endoderm overlying future anterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	2G	Expression exclusively in anterior neurectoderm.
TS11: node	Absent		2G

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		2H
TS15: somite	Present		2H
TS15: unsegmented mesenchyme	Strong	Regionally restricted	2H	High levels of expression in two stripes in presomitc mesoderm, which is about to segment.