Probe:	Fzd2 probe3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: ascending aorta	Present		2
TS20: pulmonary trunk	Present		2
TS20: ventricle cardiac muscle	Present		2
TS20: outflow tract	Present	Regionally restricted	2	Expression was detected in the mesenchyme of the outflow tract septum.
TS20: heart ventricle	Moderate	Regionally restricted	2	Moderate expression was detected in the free ventricular wall.
TS20: heart atrium	Absent		2

Structure	Level	Pattern	Image	Note
TS20: carotid artery	Present
TS20: subclavian artery	Absent
TS20: vein	Absent			The veins in the thoracic region did not shown any signal.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present		3a
TS22: pulmonary trunk	Present		3a
TS22: ventricle cardiac muscle	Present		3a
TS22: outflow tract	Present	Regionally restricted	3a	Expression was detected in the mesenchyme of the outflow tract.
TS22: visceral pericardium	Present	Regionally restricted	3a	Expression was detected in the epicardium overlying the ventricles.
TS22: heart atrium	Ambiguous		3a	The atria showed hardly any expression.
TS22: trachea	Present		3a
TS22: embryo mesenchyme	Present		3a	Expression was in muscle-forming mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: aorta	Present
TS24: descending aorta	Present
TS24: pulmonary trunk	Present
TS24: ventricle cardiac muscle	Weak	Regionally restricted		A low level of staining was detected in the trabecular myocardium in the ventricles.
TS24: heart atrium	Ambiguous			The atria showed little signal.
TS24: coronary artery	Ambiguous			The coronary arteries did not show a marked staining.
TS24: rib cartilage	Present
TS24: trachea	Present
TS24: embryo mesenchyme	Present			Expression was in muscle-forming mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: ascending aorta	Weak		4
TS26: pulmonary trunk	Weak		4
TS26: descending aorta	Absent		4
TS26: heart atrium	Absent		4
TS26: heart ventricle	Absent		4
TS26: pulmonary artery	Absent		4

Structure	Level	Pattern	Image	Note
TS26: rib cartilage	Present
TS26: trachea	Present
TS26: embryo mesenchyme	Present			Expression was in muscle-forming mesenchyme.